امکان استفاده از مارچوبه ایرانی (Asparagus persicus) در برنامه‏ های به‏ نژادی

نویسندگان

1 گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه زنجان، زنجان، ایران.

2 گروه علوم باغبانی، دانشکده تولید گیاهی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران.

3 گروه ژنتیک، دانشکده ژنتیک، دانشگاه کوردوبا، کوردوبا، اسپانیا.

چکیده

Asparagus officinalis is the most economically important species of asparagus. A. persicus is known as Iranian asparagus among the wild species which can grow under harsh environmental conditions such as high temperature and saline and dry soils. Therefore, it can be a suitable germplasm for interspecific hybridization in asparagus breeding programs. The possibility of using A. persicus in breeding programs and also employing molecular markers to detect hybrid plants developed from hybridization between A. persicus and some other species of asparagus were investigated. Twenty one accessions of asparagus from diploid (2x) to dodecaploid (12x) from six different countries were used. Successful crosses were made for A. officinalis (2x) × A. persicus (2x) by hand pollination. Hybridization rate for A. persicus was 34.1%. AG8 primer could detect specific allele for A. persicus. TC6 primer is a suitable molecular marker to detect interspecific hybrid plants for A. persicus in open pollinated and controlled crosses. ACM101 primer that has been designed for onion could also be used to evaluate genetic polymorphism in different accessions of asparagus.

کلیدواژه‌ها

عنوان مقاله [English]

The Possibility of Using Iranian Asparagus (Asparagus persicus) in Breeding Programs

نویسندگان [English]

  • M.E. Ranjbar 1
  • Z. GHaremani 1
  • S.J. Mousavizadeh 2
  • T. Barzegar 1
  • J. Gil 3
1 , respectively, Department of Horticulture, Faculty of Agriculture, University of Zanjan, Zanjan, Iran.
2 Department of Horticulture, Faculty of Plant Production, Gorgan University of Agricultural and Natural Resources Sciences, Gorgan, Iran.
3 , Department of Genetics, Faculty of Genetics, University of Cordoba, Cordoba, Spain.
چکیده [English]

Asparagus officinalis is the most economically important species of asparagus. A. persicus is known as Iranian asparagus among the wild species which can grow under harsh environmental conditions such as high temperature and saline and dry soils. Therefore, it can be a suitable germplasm for interspecific hybridization in asparagus breeding programs. The possibility of using A. persicus in breeding programs and also employing molecular markers to detect hybrid plants developed from hybridization between A. persicus and some other species of asparagus were investigated. Twenty one accessions of asparagus from diploid (2x) to dodecaploid (12x) from six different countries were used. Successful crosses were made for A. officinalis (2x) × A. persicus (2x) by hand pollination. Hybridization rate for A. persicus was 34.1%. AG8 primer could detect specific allele for A. persicus. TC6 primer is a suitable molecular marker to detect interspecific hybrid plants for A. persicus in open pollinated and controlled crosses. ACM101 primer that has been designed for onion could also be used to evaluate genetic polymorphism in different accessions of asparagus.

کلیدواژه‌ها [English]

  • Iranian asparagus
  • interspecific hybridization
  • SSR markers and wild species

مراجع

Caruso, M., Federici, C. T., and Roose, M. L. 2008. EST-SSR markers for asparagus genetic diversity evaluation and cultivar identification. Molecular Breeding 21: 195-204.
 
Doyle, J. J., and Doyle, J. L. 1990. Isolation of plant DNA from fresh tissue. Focus 12: 13-15.
 
Kanno, A., and Yokoyama, J. 2011. Asparagus. pp. 23-42. In: Chittaranjan, K. (ed.) Wild crop relatives: genomic and breeding resources. Springer.
 
Kuhl, J. C., Cheung, F., Yuan, Q., Martin, W., Zewdie, Y., McCallum, J., Catanach, A., Rutherford, P., Sink, K. C., Jenderek, M., Prince, J. P., Town, C. D., and Havey, M. J. 2004. A unique set of 11,008 onion (Allium cepa) ESTs reveals expressed sequence and genomic differences between monocot orders Asparagales and Poales. Plant Cell 16: 114-125.
 
Morais, S., Vieira, A., Almeida, L., Rodrigues, L., Melo, P., Faria, L., Melo, L., Pereira, H., and Souza, T. 2016. Application of microsatellite markers to confirm controlled crosses and assess genetic identity in common bean. Crop Breeding and Applied Biotechnology 16: 234-239.
 
Moreno, R., Espejo, J., A., Cabrera, A., Millan, T., and Gil, J. 2006. Ploidic and molecular analysis of ‘Morado de Huetor’ asparagus (Asparagus officinalis L.) population; a Spanish tetraploid landrace. Genetic Resources and Crop Evolution 53 (4): 729-736.
 
Mousavizadeh, S. J., Hassandokht, M. R., and Kashi, A. 2015. Multivariate analysis of edible asparagus species in Iran by morphological characters. Euphytica 206: 445-457.
 
Norton, J. B .1913. Methods used in breeding asparagus for rust resistance. United State Department of Agriculture, Bureau of Plant Industry. Bulletin No. 263. 84 pp.
 
Nothnagel, T., Kramer, R., Budahn, H., Schrader, O., Ulrich, D., Rabenstein, F., and Schreyer, L. 2012. Enlargement of the genetic variability of garden Asparagus (Asparagus officinalis L.) to improve resistance to biotic and abiotic stresses and quality associated compounds. Acta Horticulturae 950: 143-151
 
Sica, M., Gamba, G., Montieri, S., Gaudio, L., and Aceto, S. 2005. ISSR markers show differentiation among Italian populations of Asparagus acutifolius L. BMC Genetics 6: 17.
 
Valente, M. T., Sabatini, E., Casali, P. E., and Falavigna, A. 2012. Molecular marker assisted introgression of wild asparagus species genome into the cultivated Asparagus officinalis. Acta Horticulturae 950: 181-186.
 
 
نهال و بذر
دوره 35، شماره 2
شهریور 1398
صفحه 241-149

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