تکرارپذیری مایه‌زنی آزمایشگاهی در سنجش مقاومت میوه نارس گردو به بلایت باکتریائی و ارتباط مقاومت با فنل کل

نویسندگان

مؤسسه تحقیقات اصلاح و تهیه نهال و بذر، کرج

چکیده

خسارت اصلی بیماری بلایت باکتریایی گردو با عامل Xanthomonas arboricola pv. juglandis در اثر ریزش میوه نارس و افت کیفیت میوه رسیده است. در این تحقیق، حساسیت نسبی میوه نارس 72 رقم و ژنوتیپ بومی گردو به بلایت باکتریایی در دو سال تکرار (1391 و 1392) در شرایط آزمایشگاهی، کارآیی روش ارزیابی و ارتباط غلظت فنل کل و اندازه میوه نارس با سطوح نسبی مقاومت مطالعه شد. بر اساس نتایج تجزیه مرکب داده‌ها، 7/10 درصدارقام/ژنوتیپ‌ها حساس، 1/32 درصد نیمه‌حساس و 1/57 درصد نیمه مقاوم بودند. مقایسه میانگین نتایج دو ساله نشان داد که روش ارزیابی مورد استفاده از تکرارپذیری قابل قبولی برخوردار بود. با وجودی که بیش‌ترین غلظت فنل کل در رقم مقاوم Pedro و کم‌ترین در ژنوتیپ حساس 1-2 مشاهده شد، همبستگی بین غلظت فنل کل با مقاومت معنی‌دار نبود. بین مقاومت و اندازه میوه نارس ارتباطی مشاهده نشد که مبین حساسیت یکسان کلیه مراحل میوه نارس به این بیماری است. بر اساس این نتایج، تنوع بالایی در سطوح مقاومت میوه نارس ارقام مختلف گردو به بلایت باکتریایی وجود دارد که این تنوع با مایه‌زنی در شرایط آزمایشگاهی قابل سنجش است اما اندازه‌گیری غلظت فنل کل روش مناسبی برای پیش آگاهی سطوح مقاومت نیست.

کلیدواژه‌ها


عنوان مقاله [English]

Repeatability of Laboratory Inoculation on Assessing Susceptibility of Walnut Unripe Fruits to Bacterial Blight and Relationship Between Resistance and Total Phenols

نویسندگان [English]

  • F. Mousavi
  • M. Keshavarzi
  • R. Haghjooyan
  • A. Sobhani
چکیده [English]

Main losses of bacterial blight caused by Xanthomonas arboricola pv. juglandis on walnuts is throught unripe fruit fall and mature fruit effects. In this research, the relative susceptibility of 72 walnut cultivars and local genotypes to bacterial blight was studied in laboratory condition in years 2012 and 2013. Additionally, the efficacy of the evaluation method and correlation of resistance with unripe fruit size and total phenol concentration were investigated. Based on combined analysis of data, 10.7% of germplasm was susceptible, 32.1% moderately susceptible and 57.1% moderately resistant. Analysis of two years data means indicated that the evaluation method was repeatable. The highest phenol concentration was detected in the Pedro resistant cultivar and the lowest in the susceptible genotype 2-1, but the relation between resistance and phenl level was not significant. No correlation was found between fruit size and resistance. Based on the results, a great variation was detected in resistance of unripr fruits of different genotypes and this variation can be evaluated by inoculation in laboratory condition, but phenolic levels is not reliable in assessing blight resistance.

کلیدواژه‌ها [English]

  • juglans regia L
  • Xanthomonas arboricola pv. juglandis
  • resistance
  • total phenols
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