اثر دو پایه نیمه‌پاکوتاه OH×F87TMو پیرودوارف بر جذب آهن و خصوصیات مرتبط با سبزینگی برگ برخی ارقام تجاری گلابی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 پژوهشگر، پژوهشکده میوه‌های معتدله و سردسیری، موسسه تحقیقات علوم باغبانی، سازمان تحقیقات، آموزش و ترویج کشاورزی، کرج، ایران.

2 دانشیار، پژوهشکده میوه‌های معتدله و سردسیری، موسسه تحقیقات علوم باغبانی، سازمان تحقیقات، آموزش و ترویج کشاورزی، کرج، ایران.

3 کارشناس تحقیقاتی، پژوهشکده میوه‌های معتدله و سردسیری، موسسه تحقیقات علوم باغبانی، سازمان تحقیقات، آموزش و ترویج کشاورزی، کرج، ایران.

چکیده

از مهمترین شاخص­ها در گزینش نهائی پایه­های گلابی برای کشت در مناطق پرورش این محصول در کشور، خصوصیات مرتبط با جذب آهن و سبزینگی برگ و عدم بروز کلروز برگی در ارقام پیوند شده می­باشد. بر این اساس، پژوهش حاضر با هدف ارزیابی قدرت جذب آهن کل و میزان آهن فعال و اثرآن بر روی خصوصیات مرتبط با سبزینگی برگ در چهار رقم تجاری گلابی روی دو پایه پیرودوارف و OH×F87TM در سال 1403 در ایستگاه تحقیقات باغبانی کمالشهر کرج اجرا شد. ارقام گلابی مورد بررسی شامل چهار رقم، پکهامز­تریومف، اسپادونا، درگزی و لوئیزبون بودند. طرح آزمایشی به صورت فاکتوریل در قالب طرح بلوک­های کامل تصادفی با سه تکرار با سه عامل پایه در دو سطح، رقم در چهار سطح و موقعیت برگ در دوسطح بود. نتایج بیانگر معنی­دار بودن اثر پایه، رقم و موقعیت برگ روی صفات آهن کل، آهن فعال، کلروفیل کل،کلروفیلa و b و همچنین شاخص­های رنگ­سنجی برگ بود. عدم وجود تفاوت معنی­دار بین دو پایه مورد بررسی در رابطه با میزان آهن کل و از طرف دیگر، وجود میزان بالاتری از محتوای آهن فعال، به میزان 86/2میلی­گرم بر کیلوگرم وزن خشک برگ، درارقام پیوندی روی پایه OH×F87TM نشان­دهنده مطلوبیت نسبی این پایه در خاک­هایی با سطح متوسطی از آهک فعال بود. دامنه درصد آهن فعال برگ نسبت به آهن کل، در ارقام گلابی مورد بررسی از 19/76درصد تا 20/7درصد متفاوت بود. همچنین بیشترین محتوای کلروفیل کل در برگ­های رقم درگزی و کمترین مقدار کلروفیل کل در رقم اسپادونا در مقایسه با سایرارقام مشاهده شد. پایه، رقم و موقعیت برگ بر شاخص­های رنگ سنجی b*، a* وL* برگ اثرگذار بودند. حداقل شاخص a* و b* در ترکیب پیوندی رقم درگزی روی پایه OH×F87TM مشاهده شد، درحالی­که این ترکیب پیوندی از نظر محتوای آهن و کلروفیل کل، بیشترین میزان را دارا بود. نتایج این پژوهش نشان داد که پایه  OH×F87TM 7از توانایی مطلوبی در مقایسه با تجاری پیرودوارف در رابطه با جذب و نگهداری آهن به فرم آهن فعال برخوردار بود.

کلیدواژه‌ها


عنوان مقاله [English]

Effect of Two Semi-dwarf OH×F87TM and Pyrodwarf Rootstocks on Iron Absorption and Chlorophyll Related Characteristics of Leaves in Some Commercial Pear Cultivars

نویسندگان [English]

  • M. Bayanati 1
  • H. Abdollahi 2
  • S. M. Tavousi Naeini 3
1 Reseracher, Temperate Fruits Research Center, Horticultural Sciences Research Institute, Agricultural Research, Education and Extension Organization, Karaj, Iran.
2 . Associate Professor, Temperate Fruits Research Center, Horticultural Sciences Research Institute, Agricultural Research, Education and Extension Organization, Karaj, Iran.
3 Research Officer, Temperate Fruits Research Center, Horticultural Sciences Research Institute, Agricultural Research, Education and Extension Organization, Karaj, Iran.
چکیده [English]

Iron absorption, leaf chlorophyll content and tolerance to leaf chlorosis are among important chrachteristics in the final selection of pear rootstocks for cultivation in alkaline and semi-alkaline soils of Iran. Therefore, this study aimed to evaluate the total iron absorption potential, active iron content, and their impacts on leaf chlorophyll content in four commercial pear cultivars grafted on two OH×F87TM and Pyrodwarf rootstocks. Pear cultivars; cv. Packham's Triumph, cv. Spadona, cv. Dergazi, and cv. Louis Bonne were grafted on OH×F87TM and Pyrodwarf rootstocks. The experiment was carried-out as factorial arrangements in randomized complete block design wsith three replications and three experimental factors; rootstock at two levels, pear cultivars at four levels and leaf position at two levels. Results indicated significant effect of rootstock, cultivar, and leaf position on total iron, active iron, chlorophyll a, and b, total chlorophyll, and leaf colorimetric indices. Active iron in the leaves of pear cultivars varied from 19.76% to 20.7% of total iron. The higher active iron content in the OH×F87TM rootstock (86.32 mg kg-1 dw) suggests its adaptability to moderate level of active lime in the soil. Among the pear cultivars, cv. Dergazi had the highest total chlorophyll content, while cv. Spadona had the lowest. The rootstock × cultivar × leaf position effect on colorimetric indices, L*, a*, b*, and cv. Dergazi cultivar on OH×F87 TM rootstock showing the lowest a* and b* indices and the highest iron and total chlorophyll content.
 
Keywords: Pear, total iron, active iron, colorimetric index, chlorophyll content, SPAD index.
Introduction
Pear (Pyrus communis L.) is among the five most important fruits in the world mainly due to its high adaptability to different climatic conditions. Iran is one of the main centers of diversity of the genus Pyrus in the world, and different cultivars of common pear belonging to the species P. communis L. are well adapted to the temperate climate of this region (Abdollahi, 2010). Accordingly, the cultivation of pear trees in Iran has a history of several thousand years, and traditional orchards of this fruit tree have developed and improved over the past few decades (Abdollahi, 2010; Abdollahi, 2021). Currently, one of the main components in the development and improvement of pear orchards is usage of semi-dwarf and dwarf rootstocks with adaptability to the climatic and soil conditions of the pear production areas in Iran.
Adaptability tests of different pear cultivars and rootstocks demonstrated the impact of both on fruit yield, fruit quality and the final size of pear trees in commercial orchards (Alizadeh Fallahet al., 2022). During recent decades, Pyrodwarf rootstock has been identified as a suitable rootstock for promoting early fruiting potential and reducing tree size in pear orchards established by commercial cultivars such as Dergazi, Louise Bonne and Spadona (Abdollahi, 2021). Additionally, OH×F rootstock series, paricularly OH×F217 and OH×F87, have shown promising results for fruit yield and quality. These impacts of rootstocks express in various ways, including crown volume, vigor, and growth habit induced in the grafted cultivars. The rootstocks also affect tolerance to the alkaline soils, which in turn affects the rate of iron absorption and transfer to cultivars and ultimately leaf chlorophyll content (Fallahi et al., 2002).
Tthis study aimed to compare Pyrodwarf and OH×F87TM rootstocks effect on iron absorption and leaf chlorophyll content in different grafted pear cultivars.
 
Materials and Methods
The trees were grafted on two rootstocks, Pyrodwarf and OH×F87TM, with four fire blight tolerant commercial pear cultivars including; cv. Packham's Triumph, cv. Spadona, cv. Dargazi, and cv. Louise Bonne. The trees were seven years old at the time of evaluation. The experiment was factorial arrangements in randomized complete block design with three replications and tthere experimental factors; rootstaock at two levels, cultivar at four levels and leaf position at two levels. Each experimental plot consisted of six trees of each scion/rootstock combination. Sampling was conducted in August 2024 to assess the iron absorption characteristics; total iron and active iron, as well as leaf chlorophyll content and leaf colorimetric indices. Leaf samples were collected from the lower and upper leaves of the shoots from the current season growth. These samples were then taken to the laboratory, where the leaf chlorophyll content was determined based on the SPAD index (SPAD, DCH-52p Minolta, Japan). Total iron conten was measured colorimetrically using orthophenanthroline reagent and read at 515 nm in a spectrophotometer. The chlorophyll a and b contents, and total chlorophyll content were measured using the spectrophotometric method with 80% acetone solvent and at 663 and 645 nm wavelengths. Analysis of variance for data was performed using SAS 9.2 software. The least significant difference (LSD) test was employed for means comparison at the 5% probability level.
 
Results and Discussion
Data analysis revealed that the effects of rootstock, cultivar, and leaf position were significant on the total iron, active iron, chlorophyll a, chlorophyll b, total chlorophyll contents, and leaf colorimetric indices. Additionally, rootstock × cultivar × leaf position on these traits were significant, except for chlorophyll b. The lack of significant differences in total iron content between the two rootstocks, OH×F87TM and Pyrodwarf, suggests that rootstock OH×F87TM is suitable for soils with moderate levels of active lime. This is supported by the slightly higher active iron content in rootstock OH×F87TM compared to Pyrodwarf. Comparison of colorimetric indices revealed significant difference between two rootstocks, OH×F87TM and Pyrodwarf, in a*, b*, R, G, and B indices, with the exception of the L* index. In conclusion, the comparison between two rootstocks showed that OH×F87TM rootstock can be considered and recommended as a suitable rootstock, and even superior to Pyrodwarf, for establishing semi-dwarf pear orchards in regions with soils of moderate levels of active lime.
 
References
Abdollahi, H. 2010. Pear: botany, cultivars and rootstocks. Ministry of Agriculture, Tehran, Iran. 210 pp. (in Persian).
Abdollahi, H. 2021. Comparison of bearing and yield efficiency of commercial pear cultivars on pyrodwarf semi-dwarfing rootstock. Seed and Plant Journal, 37(4), pp.399-423 (in Persian). DOI: 10.22092/sppi.2022.356262.1254
Alizadeh Fallah, M., Yadegari, M., Davarynejad, Gh. and Nemati, S.H. 2022. The rootstock and scion interaction effects on growth and bearing characteristics of young pear trees. Journal of Horticultural Science, 36(2), pp.519-531. (in Persian). DOI: 10.22067/JHS.2022.73938.1112
Fallahi, E., Colt, W.M., Fallahi, B. and Chun, I.J. 2002. The importance of apple rootstocks on tree growth, yield, fruit quality, leaf nutrition, and photosynthesis with emphasis on Fuji. Horticultural Technology, 12(1), pp.38-44. DOI:10.21273/HORTTECH.12.1.38
 

کلیدواژه‌ها [English]

  • Total iron
  • Active iron
  • Colorimetric
  • Chlorophyll
  • Spad index
Abdollahi, H. 2010. Pear: botany, cultivars and rootstocks. Ministry of Agriculture, Tehran, Iran. 210 pp. (in Persian).
 
 
Abdollahi, H. 2021. Comparison of bearing and yield efficiency of commercial pear cultivars on pyrodwarf semi-dwarfing rootstock. Seed and Plant Journal, 37(4), pp.399-423 (in Persian). DOI: 10.22092/sppi.2022.356262.1254
 
 
Abdollahi, H. 2024. A review on cultivation history, genetic resources, selection of cultivars and improvement of tree and fruit characteristics of quince (Cydonia oblonga Mill.) in Iran. Seed and Plant Journal, 39(4), pp.493-445 (in Persian). DOI: 10.22092/spj.2024.365780.1372
 
 
Abdollahi, H. and Hassani, H. 2021. Vegetative characteristics and early bearing of some fire blight tolerant European pear cultivars on semi-dwarfing Pyrodwarf® and OH×F87TM rootstocks. Seed and Plant Journal, 37(1), pp.41-62. (in Persian). DOI:10.22092/sppi.2021.124604
 
 
Alizadeh Fallah, M., Yadegari, M., Davarynejad, Gh. and Nemati, S.H. 2022. The rootstock and scion interaction effects on growth and bearing characteristics of young pear trees. Journal of Horticultural Science, 36(2), pp.519-531. (in Persian). DOI: 10.22067/JHS.2022.73938.1112
 
 
Alonso, J.M., Gomez-Aparisi, J., Anson, J.N., Espiau, M.T. and Carrera, M. 2011. Evaluation of the OH×F selections as an alternative to quince rootstocks for pear: Agronomical performance of ‘Conference’ and ‘Doyenné du Comice’. Acta Horticulturae, 903, pp.451-456. DOI: 10.17660/ActaHortic.2011.903.61
 
 
Anonymous. 2024. Leaf analysis interpretation–apples and pears. Michgan State University. Michigan State, USA. 5 pp.
 
 
Arnon A.N. 1967. Method of extraction of chlorophyll in the plants. Agronomy Journal, 23, pp.112-121.
 
 
Botelho, R.V., Schneider, E., Machado, D., Piva, R. and Verlindo, A. 2012. Quince ‘CPP’: New dwarf rootstock for pear trees on or ganic and high density planting. Revista Brasileira deFruticultura, 34(2), pp.589-596.  DOI: 10.1590/S0100-29452012000200034
 
 
Elkins R., Bell R. and Einhorn T. 2012. Needs assessment for future US pear rootstock research directions based on the current state of pear production and rootstock research. Journal of the American Pomological Society, 66, pp.153-163.
 
 
Erdal, I. andNazlı, İ. 2019. Influence of rootstock and variety on leaf nutrient concentration
of pear grown on a nutrient-sufficient soil. KahramanmaraşSütçü İmam ÜniversitesiTarımveDoğaDergisi, 22, pp.141 – 147. DOI:10.18016/ksutarimdoga.vi.563740
 
 
Esmaeili, A., Abdollahi, H., Bazgir, M. and Abdossi, V. 2019. Effect of lime
concentration on pear’s rootstock/scion combinations. Horticultural Science, (Prague) 46, pp.123-131. DOI: 10.17221/210/2017-HORTSCI.
 
 
Faizi, Z.A., Öztürk, A. and Ullah, I. 2023. Leaf chlorophyll contents in some European pear cultivars grafted on different rootstocks and its relation with growth and yield. Spanish Journal of Agricultural Research, 21(3), pp.1-10. DOI: 0.5424/sjar/2023213-20255
 
 
Fallahi, E., Colt, W.M., Fallahi, B. and Chun, I.J. 2002. The importance of apple rootstocks on tree growth, yield, fruit quality, leaf nutrition, and photosynthesis with emphasis on Fuji. Horticultural Technology, 12(1), pp.38-44. DOI:10.21273/horttech.12.1.38
 
 
FAO. 2023. World food and agriculture-statistical yearbook. Food and Agriculture Organization Publication. Rome, Italy.
 
 
Hamouda, H.A., El-Dahshouri, M.F., Omaima, M.H. and Nagwa, G.Z. 2015.Response of le conte pear performance, chlorophyll content and active iron to foliar application of different iron sources under the newly reclaimed soil conditions. International Journal of ChemTech Research, 8, pp.1446-1453.
 
 
Ikinci, A., Bolat, I., Ercisli, S. and Kodad, O. 2014. Influence of rootstocks on growth, yield, fruitquality and leaf mineral element contents of pear cv. Santa Maria in semi-arid conditions. Biological Research, 47(1), pp.1-8. DOI: 10.1186/0717-6287-47-71
 
 
Katyal, J.C. and Sharma, B.D. 1980. A new technique of plant analysis to resolve iron chlorosis. Plant and Soil, 55, pp.105-119. DOI: 10.1007/BF02149714
 
 
Maleki Asayesh, Z., Arzani, K., Mokhtassi-Bidgoli, A., and Abdollahi, H. 2023. Enzymatic and non-enzymatic response of grafted and ungrafted young European pear (Pyrus communis L.) trees to drought stress. Scientia Horticulturae, 310, 111745, DOI: 10.1016/j.scienta.2022.111745
 
 
Manda, B., Wermund, U., Phavaphutanon, L. and Cronje, R. 2021. Tehmperate fruits: Production, processing, and marketing. Apple Academic Press Inc. Palm Bay, Florida, USA. 559 pp.
 
 
Meland, M. 2010. Performance of six European plum cultivars on four plum rootstocks growing in a northern climate. Acta Agriculturae Scandinavica Section B–Soil and Plant Science, 60(4), pp.381-387. DOI: 10.1080/09064710903103917
 
 
Meszaros, M., Lanar, L., Kosina, J. and Namestek, J. 2019. Aspects influencing the rootstock-scion performance during long term evaluation in pear orchard. Horticultural Science (Prague), 46(1), pp.1-8. DOI: 10.17221/55/2017-HORTSCI
 
 
North M., de Kock, K. and Booyse, M. 2015. Effect of rootstock on Forelle pear (Pyrus communis L.) growth and production. South African Journal of Plant and Soil, 32, pp.65-70. DOI: 10.1080/02571862.2014.981881
 
 
Rezaei, A., Abdollahi, H. and Arzani, K. 2022. Preliminary evaluation of different pear species (Pyrus spp.) based on distinctive characteristics of seed, fruit and early growth of seedlings. Seed and Plant Journal, 38(4), pp.385-408. DOI: 10.22092/spj.2023.361164. 1293
 
 
Robinson, T. 2011. High density pear production with Pyrus communis rootstocks. Acta Horticulturae, 909: 259-269. DOI: 10.17660/ActaHortic.2022.1333.50
 
 
Uçgun, K., Butar, S., Çetinbaş, M., Altındal, M. and Cansu, M. 2021. Effect of grafting on iron uptake of Farold 40, OHF333 and OHF87 pear rootstocks from the soil. Communications in Soil Science and Plant Analysis, 52(2), pp.128-135. DOI: 10.1080/00103624.2020.1854285
 
 
Zhao, M., Sun, W., Li, H., Wang, W., Cao, G. and Wang, F. 2022. The effects of the tree structure of Zaosu pear on the transport and distribution of photosynthetic assimilates and fruit quality under desert-area conditions. Agronomy, 12(10), pp.1-15. DOI: 10.3390/agronomy12102440