ارزیابی تنوع ژنتیکی برخی از ژنوتیپ‌های جنس کنار (Ziziphus) با استفاده از ویژگی‌های ریخت‌شناسی و نشانگرهای مولکولی

نویسندگان

1 دانشجوی سابق کارشناسی ارشد، دانشکده کشاورزی، دانشگاه ایلام، ایلام، ایران.

2 استادیار، گروه علوم باغبانی-دانشکده کشاورزی- دانشگاه ایلام- ایران

3 استادیار، دانشکده کشاورزی، دانشگاه ایلام، ایلام ایران.

چکیده

کنار و عناب (Ziziphus spp.) از میوه‌های گرمسیری و نیمه‌گرمسیری می‌باشند. در پژوهش حاضر 72 نمونه از پنج گونه‌ی متعلق به جنس Ziziphus که از بخش‌های مختلف استان خوزستان و ایلام جمع‌آوری شده بودند بر اساس صفات برگ و میوه ارزیابی شدند. نتایج ارزیابی خصوصیات ریختی نشان داد تنوع بالایی در برخی از صفات مانند وزن میوه، نسبت گوشت به بذر، اندازه برگ، نسبت قند به اسید میوه وجود داشت. در میان نمونه‌ها بیشترین و کمترین وزن میوه به ترتیب متعلق به دو گونه Z. mauritiana و Z. nummularia بود. دامنه تغییرات وزن میوه در این پژوهش بین 96/0 تا 46/21 گرم بدست آمد. همچنین، در بخش دوم این پژوهش 30 نمونه از میان ژنوتیپ‌های بررسی شده که دارای وزن میوه بیشتر بودند انتخاب و تنوع ژنتیکی میان آنها با 15 آغازگر ISSR بررسی شد. در مجموع 166 آلل از 15 مکان ISSR شناسایی و اندازه آلل‌ها در محدوده 170 تا 1500 جفت‌باز متغیر بود. تعداد آلل‌های مشاهده شده برای هر مکان در دامنه 6 (LBMB-B) تا 15 (HB12 و HB14) آلل با میانگین 06/11 آلل برای هر مکان بود. شاخص محتوای اطلاعات چندشکلی برای مکان‌های HB14 و UBC-826 بالا (81/0) و برای مکان LBMB-B دارای کمترین مقدار (65/0) و میانگین آن 76/0 در بین همه مکان‌های ISSR بود.

کلیدواژه‌ها

عنوان مقاله [English]

Evaluation of Genetic Diversity in Some Ziziphus Genotypes Using Morphological Characteristics and Molecular Markers

نویسندگان [English]

  • E. Norozi 1
  • Javad Erfani-Moghadam 2
  • A. Fazeli 3
1 Former M. Sc. Student, Faculty of Agriculture, Ilam University, Ilam, Iran.
2 Assistant Professor, Department of Horticulture,Faculty of Agriculture, Ilam University, Ilam, Iran.
3 Assistant Professor, Faculty of Agriculture, Ilam University, Ilam, Iran.
چکیده [English]

Ber and jujube (Ziziphus spp.) are tropical and subtropical fruits. Seventy two accessions from five Ziziphus species including: Z. jujuba, Z. nummularia, Z. spina-christi, Z. mauritiana and Z. oxyphylla collected from different parts of Khoozestan and Ilam proviences were evaluated using leaf and fruit characteristics. The primary results of morphological characteristics showed that there were high variability for some of the evaluated traits such as fruit weight, pulp:seed ratio, leaf size and TSS/TA ratio. The highest and lowest fruit weight were recorded in Z. mauritiana and Z. nummularia species. In the second experiment, 30 accessions from evaluated germplasm which had higher fruit weight were selected and their genetic diversity was investigated using 15 ISSR primers. A total of 166 alleles were identified by the 15 ISSR primers with their sizes ranging from 170 to 1500 bp. The number of observed alleles for each locus ranged from 6 (LBMB-B) to 15 (HB14, HB12) with an average of 11.06 alleles per locus. Polymorphic information content (PIC) was highest (0.81) in the HB14 and UBC-826 loci, while the LBMB-B locus had the lowest value (0.65), with an average of 0.76 among all ISSR loci.

کلیدواژه‌ها [English]

  • Ber
  • jujube
  • genetic resources
  • fruit weight
  • ISSR
  • primer

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نهال و بذر
دوره 34، شماره 2
شهریور 1397
صفحه 223-243

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