تجزیه ژنتیکی صفات زراعی در برخی ژنوتیپ‌های تیپ شرقی توتون با استفاده از تلاقی دی‌آلل

نویسندگان

1 کارشناس ارشد، دانشکده کشاورزی، دانشگاه مراغه، مراغه، ایران.

2 استاد، دانشکده کشاورزی، دانشگاه ارومیه، ارومیه، ایران.

3 استادیار، دانشکده کشاورزی، دانشگاه مراغه، مراغه، ایران.

4 استادیار،گروه مهندسی تولید و ژنتیک گیاهی، دانشکده کشاورزی، دانشگاه مراغه، مراغه، ایران

چکیده

به منظور برآورد اثر ژنتیکی برخی صفات زراعی شش ژنوتیپ توتون تیپ شرقی، در یک طرح دی‌آلل یکطرفه با یکدیگر در سال 1392 تلاقی داده شدند. در سال 1393 پانزده تلاقی نسل F1 به همراه شش لاین والدینی در مزرعه تحقیقاتی مرکز تحقیقات توتون ارومیه در قالب طرح بلوک‌های کامل تصادفی با سه تکرار کشت شدند. تعداد برگ، طول و عرض برگ، ارتفاع گیاه، قطر ساقه، فاصله میانگره، وزن تر برگ و وزن خشک برگ اندازه‌گیری و با استفاده از روش دوم گریفینگ و مدل اثر مخلوط B مورد تجزیه قرار گرفتند. نتایج حاصل از تجزیه واریانس نشان داد که بین ژنوتیپ‌ها از نظر صفات ارتفاع بوته، تعداد برگ و وزن تر برگ در سطح احتمال یک درصد و برای صفت وزن خشک برگ در سطح احتمال پنج درصد تفاوت معنی‌دار وجود داشت. ترکیب‌پذیری عمومی ژنوتیپ‌ها برای هر چهار صفت ذکر شده در سطح احتمال یک درصد معنی‌دار شد. همچنین ترکیب‌پذیری خصوصی برای ارتفاع بوته، تعداد برگ و وزن تر برگ در سطح احتمال یک درصد معنی‌دار بود.

کلیدواژه‌ها


عنوان مقاله [English]

Genetic Analysis of Agronomic Traits in Some Oriental-Type Tobacco (Nicotiana tabaccum) Genotypes Using Diallel Crossing

نویسندگان [English]

  • S. Dadashi 1
  • R. Darvishzadeh 2
  • M. Nouraein 3
  • H. Hatami Maleki 4
1 M.Sc. Faculty of Agriculture, Faculty of Agriculture, University of Maragheh, Maragheh, Iran.
2 Professor, Faculty of Agriculture, Urmia University, Urmia, Iran.
3 Associate Professor, Faculty of Agriculture, University of Maragheh, Maragheh, Iran.
4 Associate Professor, Department of Plant Production and Genetics, Faculty of Agriculture, University of Maragheh, Maragheh, Iran
چکیده [English]

To estimate genetic parameters of agronomic traits in tobacco, six oriental-type tobacco varieties were crossed based on diallel mating system without reciprocals in 2013. F1 populations and six parental lines were planted using randomized complete block design with three replications in 2014 at research field of Urmia Tobacco Research Center, Urmia, Iran. Some charectristics including number of leaves, leaf length and width, plant height, stem diameter, internodes length, fresh leaf and dry weight were measured and analyzed using Griffing method II and mixed effect model B. Analysis of variance revealed significant differences (P≤0.01) between varieties for plant height, leaf number and fresh weight and for leaf dry weight (P≤0.05). General combining ability of varieties for all aforementioned traits was significant at the 1% probability level. Specific combining ability was also significant at the 1% probbaility level for plant height, leaf number and fresh weight. Both additive and non-additive (dominance) gene effects were involved in controlling of plant height, leaf number and fresh weight, however, additive gene effect controlled leaf dry weight. In general, the ratio of GCA to SCA was significant for all of the studied traits except leaf fresh weight which emphasizes the importance of additive effect in inheritance of this trait.

کلیدواژه‌ها [English]

  • Combining ability
  • oriental-type tobacco
  • diallel crossing
  • genetic parameters
  • leaf number
  • leaf dry weight
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