ارزیابی قرابت ژنتیکی نمونه‌های جمعیتی خار مریم(Silybum marianum L.) با استفاده از نشانگرهای CDDP و SCoT

نویسندگان

1 استادیار، گروه علوم کشاورزی، دانشگاه پیام نور اصفهان، اصفهان، ایران

2 کارشناسی ارشد، گروه علوم کشاورزی، دانشگاه پیام نور اصفهان، اصفهان، ایران

3 کارشناسی ارشد، گروه علوم پایه، دانشگاه پیام نور، اصفهان، اصفهان، ایران

چکیده

خارمریم با نام علمی Silybum marianum L. گیاهی از خانواده کاسنی است که به دلیل دارا بودن متابولیت‌های ثانویه‌ی مهمی مانند سیلی‌مارین از خواص درمانی بالایی برخوردار است. با توجه به اثر درمانی قابل توجه این گیاه و لزوم کشت آن در ایران، شناخت تنوع ژنتیکی آن به منظور بهره‌گیری در برنامه‌های به‌نژادی از اهمیت بالایی برخوردار است. در تحقیق حاضر، قرابت ژنتیکی هشت نمونه جمعیتی خارمریم با استفاده از نشانگرهای CDDPو SCoT مورد بررسی قرار گرفت. بر اساس نتایج ارزیابی مولکولی، از تعداد هفت آغازگر SCoT، در مجموع 62 نوار قابل‌تشخیص به دست آمد که 53 نوار (48/85%) چندشکل بود در حالی‌که از هفت آغازگر CDDP، در مجموع 58 نوار قابل‌تشخیص به دست آمد که 50 نوار (2/86%) چندشکلی نشان دادند. میانگین شاخص محتوای چندشکل (PIC) حاصل از نشانگرهای CDDP و SCoT به ترتیب برابر با 39/0 و 23/0 بود. نتایج حاصل از تجزیه خوشه‌ای نشانگر SCoTو CDDP توانست نمونه‌های خار مریم را به سه گروه تقسیم کند. نشانگرهای CDDP و SCoT هر دو تنوع درون جمعیتی بیشتری را نسبت به تنوع بین جمعیتی نشان دادند. سطح بالای تنوع ژنتیکی مشاهده شده در نمونه‌های مورد مطالعه می‌تواند در برنامه‌های به‌نژادی آینده مورد استفاده قرار گیرد.

کلیدواژه‌ها

عنوان مقاله [English]

Assessment of Genetic Diversity in Milk Thistle (Silybum marianum L.) Accessions Using SCoT and CDDP Markers

نویسندگان [English]

  • M. Koohi- Dehkordi 1
  • A. Rafizadeh 2
  • E. Latifi 3
1 Assistant professor, Agricultural Sciences Group, Payame Nour University of Isfahan, Isfahan, Iran.
2 M. Sc. Graduate, Agricultural Sciences Group, Payame Nour University of Isfahan, Isfahan, Iran.
3 M. Sc. Graduate, Agricultural Sciences Group, Payame Nour University of Isfahan, Isfahan, Iran.
چکیده [English]

Milk Thistle (Silybum marianum L.) is a plant from the Asteraceae family that has high therapeutic properties due to its secondary metabolites, such as silymarin. Considering the significant therapeutic effects of this plant and the necessity of its cultivation in Iran, evaluation of its genetic diversity is important for breeding purposes. In the present study, the genetic relationship of eight milk thistle accessions was studied using CDDP and SCoT markers. Based on the results of the molecular evaluation, a total of 62 detectable bands were obtained from seven selective SCoT primers, of which 53 polymorphic band (85.48%) were found, while from the seven CDDP primers, a total of 58 detectable bands were obtained, of which 50 bands were polymorphic (86.2%). The mean polymorphic information content index (PIC) obtained from CDDP and SCoT markers were 0.39 and 0.23, respectively. Both the CDDP and SCoT markers showed more variation between accessions than within accessions. The high level of genetic diversity observed in the studied accessions can be used in milk thistle breeding programs.

کلیدواژه‌ها [English]

  • Genetic diversity
  • molecular marker
  • metabolite
  • Polymorphism
  • silymarin

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نهال و بذر
دوره 34، شماره 1
خرداد 1397
صفحه 63-80

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