ارزیابی مقاومت ژنوتیپ‌های لوبیا (Phaseolus vulgaris L.) به ویروس موزائیک خیار (Cucumber mosaic virus: CMV)

نویسندگان

1 گروه زراعت و اصلاح نباتات،دانشکده کشاورزی، واحد کرج، دانشگاه آزاد اسلامی، کرج.

2 گروه زراعت و اصلاح نباتات، دانشگاه تهران، کرج.

3 گروه گیاهپزشکی دانشکده کشاورزی، واحد شیراز، دانشگاه آزاد اسلامی، شیراز.

4 ، گروه گیاهپزشکی دانشکده کشاورزی، واحد شیراز، دانشگاه آزاد اسلامی، شیراز.

5 گروه علوم کشاورزی، دانشگاه پیام نور، تهران.

چکیده

به منظور ارزیابی مقاومت برخی از ژنوتیپ‌های لوبیا به بیماری ویروسی موزائیک خیار (CMV) آزمایشی در گلخانه تحقیقاتی دانشکده کشاورزی دانشگاه آزاد اسلامی شیراز انجام شد. چهل و نه ژنوتیپ مختلف لوبیا از گروه‌های لوبیا قرمز، لوبیا چیتی و لوبیا سفید در قالب طرح مربع لاتیس با دو تکرار مورد ارزیابی قرار گرفتند. ویروس موردنظر از مزارع لوبیا سبز در شیراز جمع‌آوزی، خالص‌سازی و با آزمون الایزا تائید شد. ویروس خالص شده روی گیاهچه‌های رقم حساس لوبیا تکثیر و به صورت مکانیکی با پودر کاربوراندوم برای آلوده‌سازی ژنوتیپ‌ها مورد استفاده قرار گرفت. در این بررسی درصد آلودگی ظاهری، درصد آلودگی واقعی (با آزمون الایزا) و میزان کلروفیل و سطح برگ ژنوتیپ‌ها اندازه‌گیری شد. بر اساس نتایج به دست آمده از این تحقیق، ژنوتیپ‌های لوبیا قرمز کمترین مقاومت را به این بیماری نشان دادند. همبستگی بالائی بین صفات مورد ارزیابی مشاهده شد که حاکی از تأثیر مثبت ویروس بر دستگاه فتوسنتزی و متعاقب آن تأثیر غیرمستقیم بر درصد آلودگی بود. براساس واکنش به ویروس، ژنوتیپ‌ها به چهار گروه مقاوم، نیمه‌مقاوم، نیمه حساس و حساس گروه‌بندی شدند. ژنوتیپ‌های Dehghan، Cardinal، Ks 41125، Ks 21191، Ks 21414، Ks 31137 و Capsoli به ترتیب بالاترین سطح مقاومت را داشتند.

کلیدواژه‌ها


عنوان مقاله [English]

Screening of Drought Tolerant Genotypes in Kabuli Chickpea Core Collection

نویسندگان [English]

  • S. A. Anjavi Amiri 1
  • M. R. Bihamta 2
  • S. Ghasemi 3
  • M. M. Kamelmanesh 4
  • A. Afzalifar 5
1 Department of Agronomy, College of Agriculture, Karaj Branch, Islamic Azad University, Karaj, Iran.
2 Department of Agronomy and Plant Breeding, University of Tehran, Karaj, Iran.
3 Department of Plant Protection, College of Agriculture, Shiraz Branch, Islamic Azad University, Shiraz, Iran.
4 Department of Plant Protection, College of Agriculture, Shiraz Branch, Islamic Azad University, Shiraz, Iran.
5 Agricultural Science Group, Payam-e-Nour University, Teran, Iran.
چکیده [English]

The present study was carried out on 300 Kabuli type chickpea genotypes clustered as terminal drought tolerant in chickpea core collection of National Plant Gene Bank of Iran. Genotypes were planted in Neyshabur in two separate experiments, one as control treatment (one irrigation at flowering and one at podding stages) and the other as drought treatment (rainfed) for two consecutive cropping seasons (2012-2014) using an augment design. High variation in drought tolerane and susceptibility suggested a high genetic diversity amoung the genotypes. Biplot display of genotypes in two growing seasons showed, that in 2012-13 growing season genotypes 5212, 5375, 6072, 5211, 5856, 6223, 5233, 5989, 5384, 5445, 6193 and in 2013-14 genotypes 5724, 5443, 5712, 5905, 5911, 6223, 5458, 5468 and 5856 were the most tolerant genotypes. Landrace genotypes were generally more tolerant than tolerant check cultivars such as Jam, Arman and Azad. Genotypes 6223, 5856, 5375, 5445, 5989 and 6193 were more tolerant genotypes in two years. These results demonstrated similar behavior of landrace genotypes in two different growing seasons and the stability of these genotypes despite the difference in stress intensity and makes clear the importance of valuable national chickpea genetic resources for development of pre breeding materials.

کلیدواژه‌ها [English]

  • Kabuli chickpea
  • core collection
  • landrace genotypes
  • drought tolerance
  • tolerance indices
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