ژن‌ها یا مکان‌های ژنی مرتبط با مقاومت به بیماری بلایت فوزاریومی سنبله در برخی لاین‌های امیدبخش گندم

نوع مقاله : مقاله پژوهشی

نویسندگان

1 مؤسسه تحقیقات اصلاح و تهیه نهال و بذر، سازمان تحقیقات، آموزش و ترویج کشاورزی، کرج، ایران

2 مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی استان گلستان، سازمان تحقیقات، آموزش و ترویج کشاورزی، گرگان، ایران

3 مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی استان اردبیل (مغان)، سازمان تحقیقات، آموزش و ترویج کشاورزی، اردبیل، ایران

چکیده

بیماری بلایت فوزاریومی سنبله که توسط قارچ Fusarium graminearum ایجاد می‌شود، یکی از بیماری‌های مخرب گندم در اقلیم گرم و مرطوب شمال ایران است. این بیماری علاوه بر کاهش محصول، به علت تولید مایکوتوکسین‌های مختلف توسط عامل بیماری تهدیدی جدی برای سلامت انسان و دام به شمار می‌رود. در تحقیق حاضر بیست ژنوتیپ امیدبخش گندم به همراه دو رقم شاهد سومای 3 (Sumai 3) و فلات، با استفاده از نشانگرهای مولکولی SSR از نظر وجود ژن‌ها یا مکان‌های ژنی (QTLs) مرتبط با مقاومت به بیماری بلایت فوزاریومی سنبله مورد بررسی قرار گرفتند. این ژنوتیپ‌ها از نظر مقاومت و تأیید وجود ژن‌ها یا مکان‌های ژنی مرتبط با مقاومت، در شرایط مزرعه و گلخانه نیز ارزیابی شدند. در این تحقیق، از ۴۳ نشانگر بررسی شده، ارتباط چهار نشانگر مولکولی Xbarc117، Xgwm304، Xgwm261 و Xgwm282 با مکان‌های ژنی مرتبط با مقاومت به بیماری بلایت فوزاریومی سنبله در چندین ژنوتیپ آزمایشی مشخص شد. داده‌های فنوتیپی به دست آمده نیز وجود مقاومت در این ژنوتیپ‌ها را تأیید کردند. اطلاعات به دست آمده از این بررسی می‌تواند در گزینش ژنوتیپ‌های مقاوم برای معرفی ارقام جدید و تهیه شناسنامه ارقام مفید باشد.

کلیدواژه‌ها

عنوان مقاله [English]

Genes/QTLs Associated to Fusarium Head Blight (FHB) Resistance in some Promising Lines of Wheat

نویسندگان [English]

  • A. Malihipour 1
  • M.A. Dehghan 2
  • k. Shahbazi 3
1 Seed and Plant Improvement Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran.
2 Crops and Horticultural Sciences Research Department, Golestan Agricultural and Natural Resources Research and Education Center, AREEO, Gorgan, Iran.
3 Crops and Horticultural Sciences Research Department, Ardebil Agricultural and Natural Resources Research and Education Center, AREEO, Ardebil, Iran
چکیده [English]

Fusarium head blight (FHB), caused by Fusarium graminearum, is a destructive disease of wheat in northern parts of Iran. In addition to yield loss, FHB may be a threat for human and animal health due to mycotoxin production by the pathogen causing the disease. The present study was conducted to examine SSR markers associated with resistance to FHB in selecting for resistance among twenty promising lines of wheat. The reactions of these genotypes to FHB were also determined under field and greenhouse conditions to confirm the effectiveness of the markers tested. In the present study, four out of 43 SSR markers including Xbarc117, Xgwm304, Xgwm261 and Xgwm282 were associated to FHB resistance genes/QTLs, in some of the promising lines. Phenotypic data also supported the effectiveness of these SSR markers for marker-assisted selection. The results of this study can be used for selection of resistant genotypes and providing cultivars/lines identification information in releasing new wheat cultivars.

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نهال و بذر
دوره 33، شماره 2
شهریور 1396
صفحه 153-175

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