ارزیابی تنوع ژنتیکی ژنوتیپ‏های نخود زراعی(Cicer arietinum L.) براساس صفات مورفولوژیک و نشانگرهای CDDP و SCoT

نویسندگان

گروه بیوتکنولوژی کشاورزی، دانشکده مهندسی انرژی و فناوری‌های نوین، دانشگاه شهید بهشتی، تهران

چکیده

شناسائی ژنوتیپ‌هائی با تنوع ژنتیکی در گیاهان، یکی از عوامل کلیدی برای نگهداری ژرم‌پلاسم و پتانسیل مواد اصلاحی برای آینده است. به منظور مطالعه تنوع ژنتیکی سی ژنوتیپ نخود بااستفاده از 11 نشانگر CDDP (DNA محرک محافظت شده) و 9 نشانگر SCoT (کدون‌های آغاز هدف) استفاده شد. ژنوتیپ‌ها در قالب طرح بلوک‌های کامل تصادفی باسه تکرار درمزرعه دانشگاه آزاد اسلامی واحد سنندج کاشته شدند. در طول آزمایش برخی صفات مانند عملکرد دانه، تعداد بذر درغلاف، شاخص برداشت، وزن صد دانه، عملکرد بیولوژیک گیاه و ارتفاع گیاه یادداشت‌برداری انجام شد. بر اساس نتایج، ماتریس فاصله ژنتیکی در محدوه‏ای بین 24/0 تا77/0 برای نشانگرCDDP و بین37/0 تا 88/0 برای نشانگر SCoT محاسبه شد. درصد چند شکلی نشانگرهای CDDP، 91 درصد و نشانگرهای SCoT، 88 درصد بود که تقریباً مشابه بودند. تجزیه خوشه‏ای نشانگرهای مولکولی و مورفولوژیک، ژنوتیپ‏ها را به سه گروه تقسیم‌بندی کرد. نتایج مطالعه نشان داد نشانگرهای CDDPو SCoT نسبت به دیگر روش‌ها از قدرت تفکیک پذیری بسیار بالایی در آشکارسازی تنوع و اطلاعات ژنومی برخوردار هستند. نشانگرهای CDDP و SCoTبه عنوان سیستم‌های مولکولی جدید برای ارزیابی ژرم‌پلاسم نخود قابل توصیه هستند.

کلیدواژه‌ها


عنوان مقاله [English]

Evaluation of Genetic Diversity of Chickpea (Cicer arietinum L.) Genotypes Based on Morphological Traits and CDDP and SCoT Markers

نویسندگان [English]

  • Z. Hajibarat
  • A. Saidi
  • R. Talebi
چکیده [English]

Identification of genetically resources variability is one of the key factors for conservation of germplasm and their potential of breeding for the future. In order to study the genetic diversity of chickpea, thirty genotypes were analyzed using 11 Conserved DNA-Derived Polymorphism (CDDP) and nine Start Codon Targeted (SCoT) Polymorphism markers. Genotypes were cultivated in a randomized complete block design with three replications in the Sanandaj Islamic Azad University farm. During the experiment, some morphological traits such as seed yield, biomass, harvest index, 100-seed weight, number of seeds/pod and plant height were scored. Based on the result, matrix of genetic distance ranged from 0.24 to 0.77 in CDDP and from 0.37 to 0.88 in SCoT markers. The level of polymorphism generated by CDDP markers (91%) was relatively similar to SCoT (88%) markers. Cluster analysis for CDDP and SCoT markers divided genotypes in three groups. Results of the study suggested superiority of CDDP and SCoT over other used marker techniques due to their high resolution and high variability in detection of genomic information, therefore, CDDP and SCoT are introduced as new molecular markers system for evaluation of chickpea germplasm.

کلیدواژه‌ها [English]

  • Chickpea
  • Genetic diversity
  • SCoT marker
  • CDDP marker
  • cluster analysis
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