Resistance of some Tomato (Lycopersicon esculentum) Cultivars to Root Knot Nematode (Meloidogyne javanica) Based on Microsatellite Marker

K. Mirehki,; M. Abdollahi,; M. Dehdari,

doi:10.22092/spij.2017.111218

Resistance of some Tomato (Lycopersicon esculentum) Cultivars to Root Knot Nematode (Meloidogyne javanica) Based on Microsatellite Marker

Authors

10.22092/spij.2017.111218

Abstract

Eight tomato cultivars (GH1, GH12, Ajeet, Karina, Cluster 5, Manisha, Tolstoi
and Dartom native) were screened for resistance against the root-knot nematode,
Meloidogyne javanica, under controlled environmental conditions. In greenhouse experiment, tomato seedlings were inoculated with two J2s/g soil. Eight weeks after inoculation, the reactions of the cultivars were evaluated. Based on the nematode reproduction factors, the cultivars GH12 and Ajeet evaluated as moderately resistant and GH1 and Manisha as tolerant, whereas the other cultivars were susceptible to M. javanica. In molecular studies, eight pair of microsatellite (SSR) markers were used to find markers linked to resistance to M. javanica. Second allele of the primer Tom 55-56 was the only allele in moderately resistant cultivars, whereas the second allele of Tom 47-48 found only in susceptible cultivars. These alleles showed significant correlation with number of galls produced by the nematode. The correlation coeffivients of Tom 47-48 with susceptibility and Tom 55-56 with resistance were 0.732 and -0.732, respectively. The other alleles were not related to susceptibility or resistance.

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