Evaluation of Some Characteristics of Collected Pomegranate Germplasm from Kerman Province in Iran

Document Type : Research Paper

Authors

1 Assistant Professor, Seed and Plant Improvement Institute, Agricultural Research, Education and Extension Organization, Karaj, Iran.

2 Assistant Professor, Agricultural and Natural Resources Research and Education Center of Yazd province, Agricultural Research, Education and Extension Organization, Yazd, Iran.

Abstract

To study genetic diversity in pomegranate germplasm collected from Kerman province, 94 accessions from the pomegranate collection of the National Plant Genetic Bank of Iran in Yazd were evaluated. For this purpose, 21 qualitative attributes were evaluated using the international descriptor for pomegranate. Analysis of Shannon index showed that shape of fruit base, seed color and vigour of tree had the highest, and color of one-year-old shoot, growth habit and bearing habit had the lowest diversity. Variation of Shannon index in each type of sweet, sweet-sour and sour genotypes followed similar pattern. Dendorgram of the cluster analysis divided sweet, sweet-sour and sour genotypes into five, seven, and eight groups, respectively. The results of boxplot study based on genotype origins indicated higher variation in the genetic distances of Ravar, Baft and Rafsanjan areas with other regions. The origins of the genotypes were divided into five groups using dendrogram. Ravar, Rafsanjan and Baft formed one group. Jiroft and Shahdad also belonged to a common group, and Sharababak and Bam, in a separate group. Sirjan and Chatroud regions stood individually in dendrogram. Results of cluster analysis by K-means method, using the principal components, showed that based on the Bayesian information criterion (BIC), the maximum genetic differentiation obtained at K = 10. The characteristics of each of the ten groups were studied. The results of this research in the field of genetic differentiation and germplasm grouping will be useful in future studies to identify genotypes and to select accssions with high levels of differentiation.

Keywords


Adabi Firouz Jaei, Z., Zamani, M., and Fatahi Moghadam, M. R. 2013. Study of wild and commercial pomegranates genotypes from the North of Iran using morphological traits. Journal of Plant Production 20(3): 91-109 (in Persian).
 
Beigi, F., Abdousi, V., and Ghasemi, A. A. 2012. Evaluation of some Iranian pomegranate cultivars for processing industries. Food Technology and Nutrition 9(5): 85-95 (in Persian).
 
Jombart, T., Devillard, S., and Balloux, F. 2010. Discriminant analysis of principal components: a new method for the analysis of genetically structured populations. BMC Genetics 11: 94.
 
Kazemi Alamuti, M., Ebrahimi, M. A., Zeinolabedini, M., Mardi, M., Roodbar Shojaie, T., Pezhman Mehr, M., and Poor Irandoost, H. 2013. Genetic diversity of Iranian sour pomegranate (Punica granatum L.) germplasm based on microsatellite markers. Seed and Plant Improvement Journal 29(1): 179-197 (in Persian).
 
Mars, M. 2000. Pomegranate plant material: genetic resources and breeding, a review. Options Méditerranéennes, Seri A, No. 42: 55-62.
 
Melgarejo Moreno, P., and Martinez, R. 1992. El Granado, Ediciones Mundi-Prensa. Madrid. 163 pp.
 
Melgarejo, P., Martinez, J., Martinez, F. R., Barrows, P., and Erez, A. 2004. Kaolin treatment to reduce pomegranate sunburn. Scientia Horticulturae 100(1-4): 349-353.
 
Rogers, J. S. 1972. Measures of genetic similarity and genetic distance. Studies in Genetics 7: 145-153.
 
Samadlouei, H., Azizi, M. H., and Barzagar, M. 2006. Investigation of physicochemical properties of ten varieties of Yazd pomegranate seed. Journal of Food Science and Technology 3(9): 19-26 (in Persian).
 
Shannon, C. E. 1948. A mathematical theory of communication. Bell System Technical Journal 27: 379-423.
 
Still, D. W. 2006. Pomegranates: a botanical perspective. pp:199-209. In: Seeram, N. P., Schulman, R. N. and Heber, D. (eds.) pomegranates: ancient roots to modern medicine. CRC Press Taylor and Francis Group, Boca Raton, Florida, Tatari, M., Fotouhi Ghazvini, R., Ghasemnejad, M., Mousavi, S. A., and Tabatabaii, S. Z. 2011. Morphological and biochemical characteristics of fruit in some pomegranate cultivars in climatical conditions of Saveh. Seed and Plant Improvement Journal 27(1): 69-87 (in Persian).
 
UPOV. 2012. Guidelines for the conduct of tests for distinctness, uniformity and stability: Pomegranate. International Union for the Protection of New Varieties of Plants, Geneva. 34 pp.
 
Verma, N., Mohanty, A., and Lal, A. 2010. Pomegranate genetic resources and germplasm conservation: a review. Fruit, Vegetable and Cereal Science and Biotechnology 4(Special Issue 2): 120-125.
 
Zarei, M., and Azizi, M. 2010. Evaluation of some physicochemical characteristics of six Iranian pomegranate (Punica granatum L.) cultivars at ripening stage. Journal of Horticultural Science 24(2): 175-183 (in Persian).