Microsatellite Markers Linked to the Genes Controlling Flowering Time and some Important Traits in F1 Almond Population Resulting from Controlled Crosses of ‘Tuono’ (♂) × ‘Shahrood-12’ (♀)

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Abstract

In this study flowering time and some morphological traits, nut and kernel characteristics were evaluated in a F1 almond progeny of 72 seedlings resulted from the cross between Touno (intermediate flowering) and Shahrood-12 (late flowering) cultivars. Modified-bulk segregant analysis in combination with 87 nuclear SSR markers spanning the whole almond genome and 5 chloroplastic SSR markers were used to identify molecular markers linked to flowering time in several descendants selected from the progeny. Results showed a quantitative inheritance of this trait in the progeny. The seedlings showed a wide range of flowering time between those of both progenitors and some were earlier than ‘Tuono’. Two microsatellite loci (CPPCT008 and EPPCU2584) were tightly linked to flowering traits. After construction of the genetic map of population, QTL analysis was performed for flowering time and nut and kernel characteristics. The results showed that the UDP-97403 locus had 4 and 0 cM distance from one gene loci controlling tree growth habit, nut width, nut thickness and kernel width, and BPPCT007 locus had 2 cM distance from one gene loci controlling kernel width. The results showed generally that genetic markers used in the present study are suitable tools for indirect selection of descendants with desirable flowering time and nut and kernel properties, and ulitization of these markers and marker assisted selection strategies which are less time consuming and efforts, can be successfully used in breeding programs of almond and other prunus species

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Seed and Plant Journal
Volume 29, Issue 4
February 2013
Pages 805-822

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