Evaluation of Relative Resistance of Safflower (Carthamus tintorius L.) Genotypes to Fusarium Root Rot Disease in Isfahan Province

A. Nasehi,; S. Shafizadeh,; S. Rezaie,; R. Shahsavari,

doi:10.22092/spij.2017.110983

Evaluation of Relative Resistance of Safflower (Carthamus tintorius L.) Genotypes to Fusarium Root Rot Disease in Isfahan Province

Authors

10.22092/spij.2017.110983

Abstract

Infected safflower seedlings were collected from different parts of Isfahan province and reactions of twenty one genotypes of safflower to six isolates were then evaluated in laboratory and greenhouse. A factorial experiment based on complete randomized block with three replications was used. Safflower seeds were germinated in the Lab. and after 48 hours, they were inoculated with one week old culture of F. solani isolates. Seven days after inoculation, lesion length on roots was measured. In greenhouse, safflower seeds were planted in 15 cm pots, five seedlings per each. Autoclaved wheat seeds inoculated with F. solani isolates were applied as inocula after seven days. Percentage of alived seedlings was assessed 7 and 45 days after inoculation. Statistically analyzed data showed significant difference among genotypes in laboratory and greenhouse tests. The results showed a negative correlation between lesion length on root and number of alive seedlings (r= -0.72). The most resistant genotype was pure line KW11 with 9.31 mm lesion length on roots and alive seedlings of 80% and 62.8% after 7 and 45 days, respectively. The most susceptible genotypes were Koseh cultivar and pure lines KW2 and KW3 with 13.29, 12.72 and 12.13 mm lesion length on roots and alive seedlings of 58%, 61.8% and 65.6% after 7 days and 25.6%, 28.4% and 28.4% after 45 days, respectively. Moreover, 7 days after inoculation, 14 genotypes had over 70% and 7 genotypes between 30 and 70% alive seedlings, and 40 days after inoculation, 18 genotypes had between 30 and 70% and only 3 genotypes had less than 30% alive seedlings.

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