Investigation of Fragrance Locus in some Aromatic Rice Cultivars

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Abstract

The fragrance and flavour is substantially important in rice commerce. Thereby, it is rational to consider the different aspects of this trait attentively. Hence, an investigation was conducted to study the allelic status of fragrance locus in aromatic rice cultivars Sang-tarom, Basmati 370 and Della. The F2 populations of three crosses among these cultivars were molecularly tested by random amplified polymorphic DNA marker OPAG-08 that is closely linked to rice fragrance gene and in PCR amplifies the 800 bp amplicon in aromatic cultivars, to study the inheritance of fragrance in F2 populations. Based on molecular results, the 800 bp amplicon was produced in 75% of F2 offspring suggesting that at least one identical allele exists in parental cultivars. In the other hand, the amplicon was not produced in the rest of F 2 offspring that reveals the presence of different allele(s) in that locus in parental cultivars. Therefore, regarding the segregation in F2 population, it seems that fragrance locus in these cultivars is controlled by different alleles leading to segregation in their F2 populations.

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