Enzymatic Anti-oxidative Mechanisms in Some Seedling and Clonal Commercial Pear Rootstocks in Response to Deficit Irrigation Stress

Document Type : Research Paper

Authors

Temperate Fruits Research Center, Horticultural Sciences Research Institute, Agricultural Research, Education and Extension Organization, Karaj, Iran.

Abstract

Tolerance to deficit irrigation and related mechanisms are very important characteristics of seedling and clonal pear rootstocks for successful commercialization. In this study, seedling rootstocks, Dargazi and Pyrus betulifolia Bunge, and clonal Pyrodwarf, OH×F69 and OH×F87 rootstocks for tolerance to deficit irrigation as well as enzymatic anti-oxidative mechanisms in response to the stress was evaluated under greenhouse conditions at temperate fruits research center, Horticultural Science Research Institute, Karaj, Iran, in 2018. The deficit irrigation stress applied at three levels: control, mild and severe by irrigation at 100, 75 and 50 percent of field capacity (FC), in greenhouse during 60 days. Rootstock heights, generation of hydrogen peroxide (H2O2), electrolyte leakage and the activities of superoxide dismutase (SOD), catalase (CAT) and ascorbate peroxidase (POX) were assessed at 30 and 60 days following the application of deficit irrigation stress. The results showed that Pyrodwarf rootstock had greater tolerance to deficit irrigation stress, even at 50% FC. The generation of H2O2 was between 23.7 μM g-1 of fresh weight in OH×F87 and 32.5 in P. betulifolia Bunge. However, as the deficit irrigation stress severity and duration increased, the H2O2 level enhanced to its maximum of 81.3 μM g-1 of fresh weight, and was highly correlated (r = 0.734**) with electrolyte leakage. In addition, the calculated correlation coefficients confirmed the order of increase in the activity of SOD and then CAT in response to the increase in the intensity of deficit irrigation stress. Based on the results of this study, tolerance mechanisms to deficit irrigation stress in pear rootstocks is partly attained by converting superoxide into hydrogen peroxide (H2O2), followed by preventing its accumulation.

Keywords

References

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Seed and Plant Journal
Volume 38, Issue 2
June 2023
Pages 205-222

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