Effect of Silver Nitrate and Dark on Anther Culture Efficiency of Greenhouse Bell Pepper (Capsicum annuum L.)

Document Type : Research Paper

Authors

1 Assistant Professor, Seed and Plant Improvement Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran

2 Associate Professor, Department of Plant Genetics and Breeding, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran

3 Professor, Seed and Plant Improvement Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran

Abstract

Androgenesis has great potential in plant breeding due to its high efficiency and applicability in many plant species. Anther culture is one of the most common technique to induce haploids in plant breeding programs. In this study, to optimize anther culture of bell pepper, the effects of four darkness periods and five concentration levels of silver nitrate were studied in four commercial hybrids; Kamelot, Inspiration, Plato and Californium. The results showed that increasing dark treatment from six to ten days, increased the percentage of embryogenic anthers and the percentage of regenerated plantlets. The highest percentages of embryogenic anthers and regenerated plantlets were obtained using 10 mg l-1 of silver nitrate and incubating anthers cultured in CP medium for 10 days in darkness. The results also showed that the highest percentage of embryogenic anthers (16.66%) was observed in the interaction effect between 10 mg l-1 of silver nitrate and cv. Plato. Also, among the studied concentrations of silver nitrates, the highest percentage of regenerated plantlets (11.71%) was obtained using 10 mg l-1 of silver nitrate.

Keywords


Ali, S., Khan, M. S., and Iqbal, J. 2010. Genotype independent in vitro regeneration system in elite varieties of sugarcane. Pakistan Journal of Botany 42 (6): 3783-3790.
 
Ari, E., Bedir, H., Yildirim, S., and Yildirim, T. 2016. Androgenic responses of 64 ornamental pepper (Capsicum annuum L.) genotypes to shed-microspore culture in autumn season. Turkish Journal of Biology 40: 706-717.
 
Bais, H. P., Sudha, G., and Ravinshankar, G. A. 2000a. Putrescine and AgNO3 influences shoot multiplication in vitro flowering and endogenous titres of polyamines in Chichorium intybus L. cv. Lucknow Local. Journal of Plant Growth Regulation 19: 238-248.
 
Bais, H. P., Sudha, G., Suresh, B., and Ravinshankar, G. A. 2000b. AgNO3 influences in vitro root formation in Decalepis hamiltonii Wight, Arn. Current Science 79: 894-898.
 
Barany, I., Testillano, P. S., Mityko, J., and Risueno, M. C. 2001. The switch of the microspore developmental programme in Capsicum involves HSP70 expression and leads to the production of haploid plants. International Journal of Biology Development 45: S39-S40.
 
Bhojwani, S., and Razdan, M. 1996. Plant tissue culture: Theory and practice. Revised Edition. Elsevier Science 39: 167-213.
 
Brar, M. S., Moore, M. J., Al khayri, J. M., Morelock, T. E., and Anderson, E. J. 1999. Ethylene inhibitors promote in vitro regeneration of cowpea (Vigna unguiculata L.). In Vitro Cellular and Developmental Biology-Plant 35 (3): 222-225.
 
Buyukalaca, S., Comlekcioglu, N., Abak, K., Ekbic, E., and Kilic, N. 2004. Effects of silver nitrate and donor plant growing conditions on production of pepper (Capsicum annuum L.) haploid embryos via anther culture. European Journal of Horticulture Science 69: 206-209.
 
Christensen, H. M., and Bamford, R. 1943. Haploids in twin seedlings of pepper, Capsicum annuum L. Journal of Heredity 34: 99-104.
 
Cristea, T. O., Leonte, C., Brezeanu, C., Brezeanu, M., Ambarus, S., Calin, M., and Prisecaru, M. 2012. Effect of AgNO3 on androgenesis of Brassica oleracea L. anthers cultivated in vitro. African Journal of Biotechnology 11 (73): 13788-13795.
 
Dumas de Vaulx, R., Chambonnet, D., and Pochard, E. 1981. Culture in vitro ďanthéres de piment (Capsicum annuum L.) amelioration des taux d’obtention de plantes chez différents génotypes par des traitements á +35°C. Agronomie 1: 859-864.
 
Dunlap, J. R., and Robacker, K. M. 1988. Nutrient salts promote light induced degradation of indole-3-acetic acid in tissue culture media. Plant Physiology 88: 379-381.
 
Dunwell, J. M., and Powell, W. 1987. Anther culture of Hordeum vulgare L.: A genetic study of microspore callus production and differentiation. Theoretical and Applied Genetics 74: 60-64.
 
Dunwell, J. M. 2010. Haploids in flowering plants: origins and exploitation. Plant Biotechnol Journal 8: 377-424.
 
Ercan, N., and Ayar Şensoy, F. 2011. Androgenic responses of different (Capsicum annuum L.) cultivars. Biyoloji Bilimleri Arastirma Dergisi 4: 59-61.
 
Eshbaugh, W. H. 1980. The taxonomy of the genus Capsicum (Solanaceae). Phytologia 47: 153-165.
 
FAO, 2020. The state of food and agriculture. Food and Agriculture Organization of the United Nations. Rome, Italy. 210 pp.
 
Fernandez, S., Michaux-Ferriere, N., and Coumans, M. 1999. The embryogenic response of immature embryo cultures of durum wheat (Triticum durum Desf.). Journal of Plant Growth Regulation 28:147-155.
 
Frary, A. and Sami, D. 2013. Eggplant. pp. 117-137. In: Kang, B. C., and Kole, C. (eds.) Genetics, genomics and breeding of peppers and eggplants. Vol. 2. CRC Press. Taylor and Francis Group.
 
Gammoudi, N., Pedro, T., Ferchichi, A., and Gisbert, C. 2018. Improvement of regeneration in pepper: A recalcitrant species. Invitro Cellular and Development Biology-Plant 54: 145-153.
 
George, L., and Narayanaswamy, S. 1973. Haploid Capsicum through experimental androgenesis. Protoplasma 78: 467-470.
 
George, R. A. T. 1999. Vegetable seed production. CABI Publishing. 327 pp.
 
Germanà, M. A. 2011. Anther culture for haploid and doubled haploid production. Plant Cell, Tissue and Organ Culture 104: 283-300.
 
Greenleaf, W. H. 1986. Pepper breeding. pp. 67- 134. In: Bassett, M. (ed.) Breeding of vegetable crops. AVI Publishing Company, INC.
 
Grubben, G. H. J., and Denton, O.A. 2004. Plant Resources of Tropical Africa, Vegetables. Prota Foundation/ Backhuys Publishers/CTA Wageningen, Netherlands. 161 pp.
 
Guilfoyle, T. J., Hagen, G., Li, Y., Ulmasov, T., Liu, Z. B., Strabala, T., and Gee, M. 1993. Auxin-regulated transcription. Australian Journal of Plant Physiology 20: 489-502.
 
Gyulai, G., Gémes Juhász, A., Sági, Z., Venczel, G., Pintér, P., Kristóf, Z., Törjék, O., Heszky, L., Bottka, S., Kriss, J., and Zatykó, L. 2000. Doubled haploid development and PCR analysis of F1 hybrid derived DH-2-R paprika (Capsicum annuum L.) lines. Journal of Plant Physiology 156: 168-174.
 
Habibzadeh, S., Shariatpanahi, M. E., Amiri, R., Emamifar, M., Oroojloo, M., Nematzadeh, G., Sadat Noori, S. A., and Heberle-Bors, E. 2011. Effect of 2,4-D as a novel inducer of embryogenesis in microspores of Brassica napus L. Czech Journal of Genetics and Plant Breeding 47 (3): 114-122.
 
IBPGR, 1983. Genetic resources of capsicum. International Board for Plant Genetic Resources. AGPG/IBPGR/82/12. Rome, Italy. 49 pp.
 
Irikova, T., Grozeva, S., and Rodeva, V. 2011. Anther culture in pepper (Capsicum annuum L.) in vitro. Acta Physiolgiae Plantarum 33: 1559-1570.
 
Jaiti, F. J., Benlhabib, O., Sharma, H. C., EI Jaafari, S., and EI Hadrami, I. 1999. Genotypic variation in anther culture and effect of ovary coculture in durum wheat. Plant Cell, Tissue and Organ Culture 59: 71-76.
 
Kim, M., Kim, J., Yoon, M., Choi, D. I., and Lee K. M. 2004. Origin of multicellular pollen and pollen embryo in cultured anthers of pepper (Capsicum annuum L.). Plant Cell, Tissue and Organ Culture 77: 63-72.
 
Kristiansen, K., and Andersen, S. B. 1993. Effects of donor plant, temperature, photoperiod and age on anther culture response of Capsicum annuum L. Euphytica 67: 105-109.
 
Kuo, J. S., Wang, Z. Z., Chien, N. F., Ku, S. J., Kung, M. L., and Hsu, H. C. 1973. Investigations on the anther culture in vitro of Nicotiana tabacum L. and Capsicum annuum L. Acta Botanica Sinica 15: 43-47.
 
Lashermes, P. 1992. Improved anther culture method for obtaining direct regeneration in wheat (Triticum aestivum L.). Journal of Genetic Breeding 51: 295-301.
 
Leuba, V., Le Tourneau, D., and Brown, J., 1989. Stability of phenyl acetic acid in liquid media. Journal of Plant Growth Regulation 8: 163-165.
 
Lu, R. Chen, Z., Goa, R., He, T., Wang, Y., Xu, H., Guo, G., Li, Y., Liu, Ch., and Huang, J. 2016. Genotypes-independent optimization of nitrogen supply for isolated microspore cultures in barley. BioMed Research International. DOI.Org/10.1155/2016/1801646.
 
Mele, E., Messeguer, J., and Camprubi, P. 1982. Effect of ethylene on carnation explants grown in sealed vessels. pp. 69-70. In: Fujiwara, A. (ed.) Plant tissue culture. Tokyo, Japan.
 
Mityko, J., Andrasfalvy, A., Csillery, G., and Fári. M. 1995. Anther-culture in different genotypes and F1 hybrids of pepper (Capsicum annuum L.). Plant Breeding 114: 78-80.
 
Mityko, J., and Fári, M. 1997. Problems and results of doubled haploid plant production in pepper (Capsicum annuum L.) via anther and microspore culture. Acta Horticulture 447: 281-287.
 
Mityko, J., Szabó, L., and Barnabás, B. 1999. Cholchicine induced ultra structural changes in barley and pepper microspores. Journal of Slovak Academy Science 54: 24-25.
 
Murovec, J., and Bohanec, B. 2012. Haploids and doubled haploids in plant breeding. Plant Breeding: 87-106.
 
Moubayidin, L., Di Mambro, R., and Sabatini, S. 2009. Cytokinin auxin crosstalk. Trends in Plant Science 14: 557-562.
 
Niroula, R. K., and Bimb H. P. 2009. Effect of genotype and callus induction medium on green plant regeneration from anther of Nepalese rice cultivars. Asian Journal of Plant Science 8: 368-374.
 
Ozkum Cyner, D., and Tipirdamaz, R. 2002. The effects of cold treatment and charcoal on the in vitro androgenesis of pepper (Capsicum annuum L.). Turkish Journal of Botany 26: 131-139.
 
Popova, T., Grozeva, S., Todorova, V., Stankova, G., Anachkov, N., and Rodeva, V. 2016. Effects of low temperature, genotype and culture media on in vitro androgenic answer of pepper (Capsicum annuum L.). Acta Physiologia Plantarum 38: 273.
 
Segui-Simarro, J. M., Corral-Martinez, P., Parra-Vega, V., and Gonzalez Garcia, B. 2011. Androgenesis in recalcitrant solanaceous crops. Plant Cell Reports 30: 765-778.
 
Shahvali-Kohshour, R., Moieni, and A., Baghizadeh, A. 2013. Positive effects of cold pre-treatment, iron source, and silver nitrate on anther culture of strawberry (Fragaria×ananassa Dusch). Plant Biotechnology Report 7: 481-488.
 
Song, H., Lou, Q. F., and Luo, X. D. 2007. Regeneration of doubled haploid plants by androgenesis of cucumber (Cucumis sativue L.). Plant Cell, Tissue and Organ Culture 90: 245-254.
 
Summer, A. T. 1994. Chromosome banding and identification absorption staining. pp. 59-81. In: Gosden, J. R. (ed.) Chromosome analysis protocols. Humana Press. Totowa, New Jersey.
 
Tang, Y., Li, H., Liu, J., Liu, B., and Lou, H. 2009. Callus formation from anther culture in baldam pear (Momordica charantia L.). Agriculture and Environmental Science 6 (3): 308-312.
 
Wang, Y. Y., Sun, C. S., Wang, C. C., and Chen, N. F. 1973. The induction of pollen plantlets of triticale and Capsicum annum from anther culture. Scientia Sinica 16: 147-151.
 
Yang, S. F., and Hoffman, N. E. 1984. Ethylene biosynthesis and its regulation in higher plants. Annual Review of Plant Physiology 35: 155-189.