Evaluation of Salinity Tolerance in Six Olive (Olea europaea L.) Genotypes in Controlled Environment

Authors

Temperate Fruit Research Center, Horticultural Sciences Research Institute, Agricultural Research, Education and Extension Organization, Karaj, Iran.

Abstract

Limitation of water resources and salinity of water and soil are important factors in the development of olive cultivation and production. Therefore, it is very important to select and introduce salinity tolerant olive cultivars. This study was carried out using six olive cultivars and promising genotypes (Conservolia, Koroneiki, Amin, D1, Ds8 and Ozineh2) and three salinity treatment (0, 50, 200 mMl-1 NaCl) as factorial experiment in completely randomized design with three replications in controlled environment. The results showed that with increasing salinity, the Na+ content increased in roots, stems and leaves tissues. The concentration of Na+, Cl- and K+ in these tissues was affected by salinity levels and olive genotypes. As the salt concentration increased, the potassium content and K+/Na+ ratio significantly decreased in roots, stems and leaves tissues. Transfer of Cl- from root to stem and leaves in susceptible cultivars and genotypes was higher than in tolerant genotypes. Cultivar Conservolia and genotypes and D1 genotype had the lowest leaf area. Salt injury index of showed that cv. Amin and Ds8 genotype had no injury symptom. However cv. Conservolia and D1 genotype showed the greatest injury symptoms by salinity stress. According to the results of this research, cv. Amin and Ds8 genotype were identified as tolerant, Ozineh2 promising genotype relatively susceptible, cv. Koroneiki as susceptible, and cv. Conservolia and D1 genotype as very susceptible to salt stress.

Keywords


Benlloch, M., Arboleda, F., Barranco, D., and Fernadez-Escobar, R. 1991. Response of young olive trees to sodium and boron excess in irrigation water. HortScience 26: 867–870.
 
Bongi, G., and Loreto, F. 1989. Gas-exchange Properties of salt stressed olive (Olea europaea L.) leaves. Plant Physiology 90: 1408–1416.
 
Bracci, T., Minnocci, A., and Sebastiani, L. 2008. In vitro olive (Olea europaea L.) cvs. Frantoio and Moraiolo microshoot tolerance to NaCl. Plant Biosystem 142: 563–571.
 
Chartzoulakis, K., Loupassaki, M. Bertaki, M., and Androulakis, I. 2002. Effects of NaCl salinity on growth, ion content and CO2 assimilation rate of six olive cultivars. Scientia Horticulturae 96: 235-247.
 
Chartzoulakis, K., Loupassak, M., and Bertaki, M. 2006a. Response of 12 olive cultivars to NaCl salinity. pp. 403-406. In: Proceedings of Olivebioteq, 2006. Marsala-Mazzara del Vallo, Italy.
 
Charzolakis, K., Psarras, G., Vemmos, S., Loupassaki, M., and Bertaki, M. 2006b. Response of two olive cultivars to salt stress and potassium supplement. Journal of Plant Nutrition 29: 2063–2078.
 
Flowers, T. J., and Yeo, A. R. 1988. Ion relations of salt tolerance. pp. 392–413. In: Baker, D., Halls J. (eds) Solute transport in plant cells and tissues. Harlow: Longman.
 
Gregorio, G. B., Senadhira, D., and Mendoza, R. D. 1997. Screening rice for salinity tolerance. IRRI discussion papers No. 22. International Rice Research Institute. Manila (Philippines). 31 pp.
 
Gucci, R., and Tattini, M. 1997. Salinity tolerance in olive. Journal of the American Society for Horticultural Science 21: 177–214.
 
Gupta, P. K. 2000. Soil, plant, water and fertilizer analysis. Agrobios. New Dehli, India. 350 pp. Fernandez, J., 2014. Understanding olive adaptation to abiotic stresses as a tool to increase crop performance. Environmental and Experimental Botany 103: 158–179.
 
Imami, A. 1996. Plant decomposition methods. Publication No. 928. Soil and Water Research Institute. Agriculture research, Education and Extension organization Press. 128 pp. (in Persian).
 
Loreto, F., and Bongi, G. 1987. Control of photosynthesis under salt stress in olive. pp. 411–420. In: Prodi, F., Rossi, F., Cristoferi, G. (eds.) Proceedings of the International Conference on Agro-meteorology.Cesena, Italy.
 
Loupassaki, M. H., Chartzoulakis, K., Sdigalaki, N. B., and Androulakis, I. I. 2002. Effects of salt stress on concentration of nitrogen, phosphorus, calcium, magnesium and sodium in leaves, shoots and roots of six olive cultivars. Journal of Plant Nutrition 25: 2457–2482.
 
Marschner, H. 1995. Mineral nutrition of higher plants. Academic Press. London. 889 pp.
 
Mousavi, S., Regni, L., Bocchini, M., Mariotti, R., Cultrera, N., Mancuso, S., Googlani, J., Chakerolhosseini, M. R., Guerrero, C., Albertini, E., Baldoni, L., and Proietti, P. 2019.
 
Physiological, epigenetic and genetic regulation in some olive cultivars under salt stress. Scientific Reports 9: 1093, doi:org/10.1038/s41598-018-37496-5.
 
Munns, R., and Termaat, A. 1986. Whole-plant responses to salinity. Australian Journal of Plant Physiology 13: 143–160.
 
Munns, R., and Tester, M. 2008. Mechanisms of salinity tolerance. Annual Review of Plant Biology 59: 651–681.
 
Olyaei, F., Baninasab, B., Ghobadi, C., and Gholami, M. 2015. Ion content and its correlation with some physiological parameters in olive cultivars in response to salinity. Iran Agricultural Research 34 (2): 61-70.
 
Regni, L., Marco Del Pino, A., Mousavi, S., Palmerini, C. A., Baladoni, L., Mariotti, R., Malrech, H., Gardi, T., Damato, R., and Proietti, P. 2019. Behavior of four olive cultivars during salt stress. Plant Science 10: 1-9.
 
Rossi, L., Francini, A., Minnocci, A., and Sebastiani L. 2015. Salt stress modifies apoplastic barriers in olive (Olea europaea L.): a comparison between a salt-tolerant and a salt-sensitive cultivar. Scientia Horticulturae 192: 38–46.
 
Roy, S. J., Negrao, S., and Tester, M. 2014. Salt resistant crop plants. Current Opinion in Biotechnology 26: 115–124.
 
Tabatabaei, S. J. 2006. Effects of salinity and N on the growth, photosynthesis and N status of olive (Olea europaea L.) trees. Scientia Horticulturae 108: 432–438.
 
Tattini, M. 1994. Ionic relations of aeroponically-grown olive genotypes during salt stress. Plant and Soil 161: 251–256.
 
Tattini, M., Gucci, R., Coradeschi, M. A., Ponzio, C., and Everard, J. D. 1995. Growth, gas exchange and ion content in Olea europaea plants during salinity stress and subsequent relief. Physiolegia Plantarum 95: 203–210.
 
Zarei, M., Azizi, M. Rahemi, M., and Tehranifar, A. 2016. Assessment of salinity tolerance of three fig cultivars based on growth and physiological factors and ions distribution. Iranian Journal of Horticultural Science and Technology 17 (2): 247-260 (in Persian).
 
Zeinanloo, A. A., 2010. Olive cultivars for oil and table olive. Saie Gostar Publications. Iran. 88 pp. (in Persian).
 
Zeinanloo, A. A., 2018. Evaluation and selection of superior olive genotypes with high oil and yield. Iranian Journal of Horticultural Science and Technology 19 (2):171-184 (in Persian).