Freezing Tolerance in Some Lentil Genotypes under Controlled Conditions

Authors

1 Research Center for Plant Sciences, Ferdowsi University of Mashhad, Mashhad, Iran.

2 Faculty of Agriculture and Research Center for Plant Sciences, Ferdowsi University of Mashhad, Mashhad, Iran.

3 Department of Agronomy, Payam-e-Noor University, Tehran, Iran.

4 Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran.

Abstract

To study freezing tolerance of 40 genotypes of lentil (Lens culinaris Medik.) an experiment as factorial arrangements in completely randomized design with three replications was carried out at the research center for plant science, Ferdowsi University of Mashhad, Mashhad, Iran, in 2017-2018. Genotypes were exposed to three levels of freezing temperatures (-13, -15 and -18 °C). The results showed that at -13, -15 and -18 °C temperature levels, 100, 93 and 30 percent of genotypes had no significant difference with their maximum survival (100%), respectively. Only three genotypes MLC12, MLC17 and MLC95 were able to maintain 100% survival at all three freezing temperature levels. The lowest temperatures reducing 50% of leaf area (RLAT50) and dry matter (RDMT50) were -16.5 and -16.7 °C, respectively, and most of the genotypes succeeded in retaining 50 percentage of these traits during the recovery period. MLC8 and MLC286 genotypes with RDMT50 of -16.7 °C had the highest ability to maintain dry weight, whereas MLC74 genotype with RDMT50 of -13.4 °C had the least cold tolerance in respect to dry weight during recovery. Generally, 37 genotypes had reasonable level of tolerance to freezing stress. Therefore, based on the results of this experiment these 37 genotypes can be used in lentil breeding program for target areas with a minimum temperature of -15 °C.

Keywords


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