Evaluation of Drought Tolerance in Promising Barley Lines under Controlled and Field Conditions

Authors

1 Faculty of Agriculture, Karaj Branch, Islamic Azad University, Karaj, Iran.

2 Seed and Plant Improvement Institute, Agricultural Research, Education and Extension Organization, Karaj, Iran.

3 Department of Agronomy and Plant Breeding, Karaj Branch, Islamic Azad University, Karaj, Iran.

Abstract

To evaluate the response of promising barley lines to drought stress, two experiments were carried out separately under controlled and field conditions. Under controlled conditions, 25 barley genotypes were tested at three levels of osmotic stress, zero (control), -5 and -10 MPa using polyethylene glycol 6000 using factorial arrangements in randomized complete block design with three replications. Analysis of variance, for this experiment, showed that the effects of genotype, osmotic potential and osmotic potential × genotype interaction were significant on germination percentage, germination rate, coleoptile length, radical weight and seed vigor index. With increasing osmotic stress, all germination related traits significantly reduced. Under field conditions, 20 barley genotypes were evaluated using randomized complete block design with three replications, under two conditions; terminal drought stress (irrigation cut off at 50% heading) and non-stress in 2016-17 and 2017-2018 cropping seasons. Combined analysis of variance showed that there was significant (P < 0.01) difference among genotypes for grain yield in stress and non-stress conditions. Mean comparison under non-stress conditions showed that the highest grain yield belonged to genotype 16, and under drought stress conditions also genotypes 16 had the highest grain yield. In this experiment, different drought tolerance and susceptibility indices were calculated using grain yield data in stressed and non-stress conditions. Based on correlation between different indices and grain yield in two conditions, the mean productivity (MP), geometric mean productivity (GMP), stress tolerance index (STI) and harmonic mean (HM) were identified as the suitable indices. According to these indices, genotypes 16, 11 and 4 were identified as the superior genotypes in both conditions. Correlation analysis between traits at germination stage with different indices showed that only radicle weight had positive and significant relationship with MP, GMP, HM and STI indices.

Keywords

References

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Seed and Plant Journal
Volume 36, Issue 2
June 2020
Pages 161-182

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