Comparison of Necrotrophic Behaviour and Electrolyte Leakage of Resistant and Tolerant Pear (Pyrus communis L.) Cultivars to Mutant Strains of Causal Agent of Fire Blight Disease under In Vitro Conditions

Authors

1 Ph. D. Student, Department of Horticulture, Abhar Branch, Islamic Azad University, Abhar, Iran.

2 Associate Professor, Temperate Fruits Research Center, Horticultural Sciences Research Institute, Agricultural Research, Education and Extension Organization, Karaj, Iran.

3 Assistant Professor, Department of Horticulture, Abhar Branch, Islamic Azad University, Abhar, Iran.

Abstract

Response of pear cultivars to causal agent of fire blight diseases is expressed by both resistance and tolerance, however the difference of the two is not clear. To better understand the mechanisms of resistance and tolerance of pear cultivars to the disease, the necrotrophic behaviour and electrolyte leakage of resistant (Dargazi) and tolerant (Harrow Sweet) cultivars was compared with susceptible cultivar (Barttlet) after inoculation by wild type and mutant strains (hrpN-, dspA/E- and hrpW-) of the bacterium. The appearance of symptoms in reaction to wild type strains occurred after three days in susceptible and tolerant cultivars, and in seven days in resistant cultivar. However, in resistant cultivar, Dargazi, disease progress slowed down. In all cultivars, one to two days before necrosis, electrolyte leakage increased to ~70%, and with the completion of necrosis it reached to 100%. The mutant strain hrpW- did not affect incidence of necrosis and electrolyte leakage, indicating no effect of this protein on resistance mechanisms. Using strain hrpN- increased the necrosis and electrolyte leakage in resistant cultivar and delayed these indices in tolerant cultivar in comparison with susceptible cultivar. Also, dspA/E- strain accelerated necrosis and electrolyte leakage in resistant cultivar in comparison with tolerant cultivar, and had no symptoms on susceptible cultivar. Considering the dual role of HrpN in pathogencity and stimulation of host defense system, the results indicated key role of HrpN protein on acquired defense system (ADS) in relation to the pathogenic role in the resistant cultivar (Dargazi) and its inverse relationship in tolerant cultivar (Harrow Sweet). This dual behaviour can be used as a tool for deeper understanding of the fire blight resistance mechanisms in pear, and also as an index for distinctness of resistant and tolerant pear cultivars.

Keywords

References

Abdollahi, H. 2003. Molecular biology of interaction between Erwinia amylovora and pear (Pyrus communis L.) genotypes with different susceptibility to fire blight. Ph.D. Thesis, Faculty of Agriculture, University of Florence, Italy. 200 pp.
 
Abdollahi, H., and Salehi, Z. 2017. Histology of oxidative stress and generation of reactive oxygen species against progress of fire blight causal agent in pear cultivars. Seed and Plant Improvement Journal 33-2: 139-162 (in Persian).
 
Abdollahi, H., Ghahremani, Z., and Erfaninia, K. 2015. Role of electron transport chain of chloroplasts in oxidative burst of interaction between Erwinia amylovora and host cells. Photosynthesis Research 124: 231-242.
 
Abdollahi, H., Rugini, E., Ruzzi, M., and Muleo R. 2004. In vitro system for studying the interaction between Erwinia amylovora and genotypes of pear. Plant Cell Tissue and Organ Culture 79: 90-95.
 
Azarabadi, S., Abdollahi, H., Torabi, M., Salehi, Z., and Nasiri, J. 2017. ROS generation, oxidative burst and dynamic expression profiles of ROS-scavenging enzymes of superoxide dismutase (SOD), catalase (CAT) and ascorbate peroxidase (APX) in response to Erwinia amylovora in pear (Pyrus communis L). European Journal of Plant Pathology 147: 279-294.
 
Bogdanove, A. J., Kim, J. F., Wei, Z., Kolchinsky, P., Charkowski, A. O., Conlin, A. K., Collmer, A., and Beer, S. V. 1998. Homology and functional similarity of an hrp-linked pathogenicity locus, dspEF, of Erwinia amylovora and the avirulence locus avrE of Pseudomonas syringae pathovar tomato. National Academy of Sciences 95: 1325-1330.
 
Brisset, M. N., and Paulin, J. P. 1991. Relationships between electrolyte leakage from Pyrus communis and virulence of Erwinia amylovora. Physiological and Molecular Plant Pathology 39: 443-453.
 
DebRoy, S., Thilmony, R., Kwack, Y.B., Nomura, K., and He, S. Y. 2004. A family of conserved bacterial effectors inhibits salicylic acid-mediated basal immunity and promotes disease necrosis in plants. National Academy of Sciences 101: 9927-9932.
 
Dong, H., Delaney, T. P., Bauer, D. W., and Beer, S. V. 1999. Harpin induces disease resistance in Arabidopsis through the systemic acquired resistance pathway mediated by salicylic acid and the NIM1 gene. Plant Journal 2: 207-215.
 
Erfaninia, K., Abdollahi, H., and Khosroshahli, M. 2014. Effect of several chloroplast electron transport chain inhibitors on interaction of Erwinia amylovora with susceptible and tolerant apple and pear cultivars. Plant Pathology 49: 201-214. (in Persian).
 
Filek, M., Walas, S., Mrowiec, H., Rudolphy-Skorska, E., Sieprawska, A., and Biesaga-Koscielniak, J. 2012. Membrane permeability and micro and macro element accumulation in spring wheat cultivars during the short-term effect of salinity- and PEG-induced water stress. Acta Physiologiae Plantarum 34: 985-995.
 
Gaudriault, S., Malandrin, L., Paulin, J. P., and Barny, M.-A. 1997. DspA, an essential pathogenicity factor of Erwinia amylovora showing homology with AvrE of Pseudomonas syringae, is secreted via the Hrp secretion pathway in a DspB dependent way. Molecular Microbiology 26: 1057-1069.
 
Hassani, M., Salami, S. A., Nasiri, J., Abdollahi, H., and Ghahremani, Z. 2016. Phylogenetic analysis of PR genes in some pome fruit species with the emphasis on transcriptional analysis and ROS response under Erwinia amylovora inoculation in apple. Genetica 144: 9-22.
 
Holtappels, M., Noben, G. P., Van Dijck, P., and Valcke, R. 2018. Fire blight host-pathogen interaction: proteome profiles of Erwinia amylovora infecting apple rootstocks. Scientific Reports 8: 11689.
 
Krasuska. U., and Gniazdowska, A. 2012. Nitric oxide and hydrogen cyanide as regulating factors of enzymatic antioxidant system in germinating apple embryos. Acta Physiologiae Plantarum 34: 683-692.
 
Leblay, C., Chevreau, E., and Robin, L. M. 1991. Adventitious shoot regeneration from in vitro leaves of several pear cultivar (Pyrus communis L.). Plant Cell, Tissue and Organ Culture 25: 99-105.
 
Narayanasamy, P. 2008. Molecular biology in plant pathogenesis and disease management: disease management. Springer. 246 pp.
 
Oh, C. S. 2005. Characterization of HrpN-interacting proteins from plants, the Hrp pathogenicity island of Erwinia amylovora, and its proteins that affect the hypersensitive response. Ph.D. Cornell University, Ithaca, NY. 334 pp.
 
Oh, C. S., Kim, J. F., and Beer, S. V. 2005. The Hrp pathogenicity island of Erwinia amylovora and the identification of three novel genes required for systemic infection. Molecular Plant Pathology 6: 125-138.
 
Oh, C. S., and Beer, SV. 2005. Molecular genetics of Erwinia amylovora involved in the development of fire blight. Federation of European Microbiological Societies Microbiology Letters 253: 185-192.
 
Sairam, R. K., and Srivastava. G. C. 2001. Water stress tolerance of wheat Triticum aestivum L.: Variation in hydrogen peroxide accumulation and antioxidant activity in tolerant and susceptible genotype. Journal of Agronomy and Crop Science 186: 63-70.
 
Taheri Shahrestani, A., Abdollahi, H., Yakhchali, B., Mehrabi, R., and Eini Gandomani, O. 2017. Comparison of the effects of Erwinia amylovora effector proteins on pear cultivars in active and inactive chloroplastic electron transport chain conditions. Modern Genetics Journal 3: 333-345.
 
Van der Zwet, T., and Keil, H. L. 1979. Fire blight: A bacterial disease of rosaceous plants. Handbook No. 510. United State Department of Agriculture. Washington DC, USA. 211 pp.
 
Vanneste, J. L. 2000. Fire Blight: The disease and its causative agent, Erwinia amylovora. CABI Publishing, Wallingford, UK. 370 pp.
 
Venisse, J. S., Barny, M. A., Paulin, J. P., and Brisset, M. N. 2003. Involvement of three pathogenicity factors of Erwinia amylovora in the oxidative stress associated with compatible interaction in pear. FEBS Letters 537: 198-202.
 
Venisse, J. S., Gullner, G., and Brisset, M. N. 2001. Evidence for the involvement of an oxidative stress in the initiation of infection of pear by Erwinia amylovora. Plant Physiology 125: 2164-2172.
 
Xie, Z., and Chen, Z. 2000. Harpin-induced hypersensitive cell death is associated with altered mitochondrial functions in tobacco cells. Molecular Plant-Microbe Interactions 13: 183-190.
 
 
Seed and Plant Journal
Volume 35, Issue 2
August 2019
Pages 137-152

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