Designing Molecular Marker Specific to PET1 Male Sterile Cytoplasm in Sunflower

Document Type : Research Paper

Authors

1 Tarbiat Modares university, Tehan Iran

2 Seed and Plant Improvement Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran.

3 Department of Plant Breeding, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran.

Abstract

Cytoplasmic-genetic male sterility is widely used for production of sunflower hybrid seeds. In sunflower, an ORF, ORFH522, presented at 3′ end of atpA gene cause male sterile phenotype. This investigation was carried out to find the molecular marker for PET1 cytoplasmic-genetic male sterility system in sunflower. To this end, five male sterile, 24 maintainer and 32 restorer lines were used as genetic materials. The gene sequences for both male sterile and male fertile were extracted from NCBI and upon their alignment, a primer pair was designed based on their different regions. The primer pair amplified a fragment of 182 bp in cytoplasmic male sterile lines, while no amplification in male fertile ones was observed; Thus, the primer is considered as a marker for male sterile cytoplasm. Also it was confirmed that all restorer lines have male sterile cytoplasm. Thus, the primer can be used efficiently in breeding programs of sunflower either in transferring male sterile cytoplasm to elite lines, or in restorer line production and purification.

Keywords

References

Balk, J., and Leaver, C. J. 2001. The PET1-CMS mitochondrial mutation in sunflower is associated with premature programmed cell death and cytochrome c release. The Plant Cell 13: 1803-1818.
 
Chase, C. D. 2007. Cytoplasmic male sterility: a window to the world of plant mitochondrial-nuclear interactions. Trends in Genetics 23:81-90.
 
Chen, L., and Liu, Y. G. 2014. Male sterility and fertility restoration in crops. Annual Review of Plant Biology 65: 579-606.
 
Gong, L., Li, C., Capatana, A., Feng, J., Qi, L., Seiler, G. J., and Jan, C. C. 2014. Molecular mapping of three nuclear male sterility mutant genes in cultivated sunflower (Helianthus annuus L.). Molecular Breeding 34: 159-166.
 
Horn, R. 2002. Molecular diversity of male sterility inducing and male-fertile cytoplasm in the genus Helianthus. Theoretical and Applied Genetics 104: 562-570.
 
Horn, R., Köhler, R. H., and Zetsche, K. 1991. A mitochondrial 16 kDa protein is associated with cytoplasmic male sterility in sunflower. Plant Molecular Biology 17: 29-36.
 
Kaul, M. L. H. 1998. Male Sterility in Higher Plants: Springer-Verlag Berlin Heidelberg. 1005 p. Kofer, W., Glimelius, K., and Bonnett, H. T. 1991. Modifications of mitochondrial DNA cause changes in floral development in homeotic-like mutants of tobacco. The Plant Cell 3: 759-769.
 
Köhler, R. H., Horn, R., Lössl, A., and Zetsche, K. 1991. Cytoplasmic male sterility in sunflower is correlated with the co-transcription of a new open reading frame with the atpA gene. Molecular and General Genetics 227: 369-376.
 
Laver, H. K., Reynolds, S. J., Moneger, F., and Leaver, C. J. 1991. Mitochondrial genome organization and expression associated with cytoplasmic male sterility in sunflower (Helianthus annuus). The Plant Journal 1: 185-193.
 
Leclercq, P. 1969. Une stérilité male cytoplasmique chez le Tournesol (Helianthus annuus L.). Annals Amélior Plant 19: 99-106 (in French).
 
Li, J. T., Yang, J., Chen, D. C., Zhang, X. L., and Tang, Z. S. 2007. An optimized mini-preparation method to obtain high-quality genomic DNA from mature leaves of sunflower. Genetics and Molecular Research 6: 1064-1071.
 
Moneger, F., Smart, C. J., and Leaver, C. J. 1994. Nuclear restoration of cytoplasmic male sterility in sunflower is associated with the tissue-specific regulation of a novel mitochondrial gene. The EMBO Journal 13: 8-17.
 
Nakai, S., Noda, D., Kondo, M., and Terachi, T. 1995. High-level Expression of a Mitochondrialorf 522 Gene from the Male-sterile Sunflower is Lethal to E. coli. Japanese Journal of Breeding 45: 233-236.
 
Pérez-Vich, B., Berry, S. T., Velasco,L., Fernandez-Martinez, J. M., Gandhi, S., Freeman, C., Heesaker, A., Knapp, S. J., and Leon, A. J. 2005. Molecular mapping of nuclear male sterility genes in sunflower. Crop Science 45: 1851-1857.
 
Sabar, M., Gagliardi, D., Balk, J., and Leaver, C. J. 2003. ORFB is a subunit of F1F(O)-ATP synthase: insight into the basis of cytoplasmic male sterility in sunflower. EMBO Reports 4: 381-386.
 
Schnable, P. S., and Wise, R. P. 1998. The molecular basis of cytoplasmic male sterility and fertility restoration. Trends in Plant Science 3: 175-180.
 
Schnabel, P. S., Engelmann, U., and Horn, R. 2008. Development of markers for the use of the PEF1 cytoplam in sunflower hybrid breeding. Plant Breeding 127: 587-591.
 
Serieys, H., and Vincourt, P. 1987. Characterization of some new CMS sources from Helianthus genus. Helia 10: 9-13 Serieys, H. 1999. Identification, study, and utilization in breeding programs of new CMS sources. FAO progress report. Helia 22 (special issue): 71-84.
 
Yue, B., Vick, B. A., Cai, X., and Hu, J. 2010. Genetic mapping for the Rf1 (fertility restoration) gene in sunflower (Helianthus annuus L.) by SSR and TRAP markers. Plant Breeding 129: 24-28. Zubko, M. K. 2004. Mitochondrial tuning fork in nuclear homeotic functions. Trends in Plant Science 9: 61-64.
 
 
Seed and Plant Journal
Volume 33, Issue 4
March 2018
Pages 475-485

Files

Share

How to cite

Statistics