Response of Pear Cultivars to Invasion of Mutant Strains of Fire Blight Causal Agent (Erwinia amylovora) Based on H2O2 Generation

Authors

1 respectively, Department of Plant Protection, Zanjan University, Zanjan, Iran.

2 Horticultural Sciences Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran.

3 Industerial and Environmental Biotechnology Department, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran.

4 Seed and Plant Improvement Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran.

Abstract

Pear (Pyrus communis), is the most important host of fire blight disease, and the response of pear cultivars to this disease is highly dependent on the host response related to Reactive Oxygen Species (ROS) generation especially Hydrogen Peroxide (H2O2). In order to have a better understanding of these reactions, quantitative level of H2O2 generation related to tissue response of pear cultivars during interaction with wild type Erwinia amylovora and mutant strains of this bacterium including HrpW–, HrpN– and DspA/E through active and inactive chloroplast was determined in present study. Comparison of results of evaluated indices including necrosis development and severity and percent of H2O2 production in periods of time after inoculation showed that HrpN and DspA/E effector proteins had the highest and HrpW effector protein had the lowest portion in pathogenicity and H2O2 production in host and HrpN had a key role during interaction with host chloroplast. Quantitative evaluation of the bacterial effector proteins on H2O2 generation indicates that HrpN and DspA/E effector proteins hahadve a complementary role in development of oxidative burst in pear cultivars and it seems that oxidative tension of host is caused by HrpN and due to complementary role of DspA/E it reaches to a destructive level for host tissue. Based on severity index of H2O2 Production, just development of this ROS in host is not enough for causing necrosis and increasing of its severity upper than index 3 is essential for necrosis and sensitivity.

Keywords

References

Abdollahi, H. 2003. Molecular biology of interaction between Erwinia amylovora and pear (Pyrus communis L.) genotypes with different susceptibility to fire blight. Ph.D. Thesis, Faculty of Agriculture, University of Florence, Italy.
 
Abdollahi, H., Ghahremani, Z., and Erfaninia, K. 2015. Role of electron transport chain of chloroplasts in oxidative burst of interaction between Erwinia amylovora and host cells. Photosynthesis Research 124: 231-242.
 
 
Abdollahi, H., Rugini, E., Ruzzi, M., and Muleo, R. 2004. In vitro system for studying the interaction between Erwinia amylovora and genotypes of pear. Plant Cell Tissue and Organ Culture 79: 90-95.
 
 
Anonymous 2013. Food and Agriculture Organization of the United Nations. http: //faostat.fao.org/
 
 
Asada, K. 2006. Production and scavenging of reactive oxygen species in chloroplasts and their functions. Plant Physiology 141: 391-396.
 
Asselbergh, D., De Vleesschauwer, D., and Hofte, M. 2008. Global switches and fine-tuning: ABA modulates plant–pathogen defence. Molecular Plant-Microbe Interactions 21: 709-719.
 
 
Azarabadi, S., Abdollahi, H., Torabi, M., Salehi, Z., and Nasiri, J. 2016. ROS generation oxidative burst and dynamic expression profiles of ROS-scavenging enzymes of superoxide dismutase (SOD) catalase (CAT) and ascorbate peroxidase (APX) in response to Erwinia amylovora in pear (Pyrus communis L.). European Journal of Plant Pathology 147: 279-294.
 
 
Baker, C. J., and Orlandi, E. W. 1995. Active oxygen in plant pathogenesis. Annual Review of Phytopathology 33: 299-321.
 
 
Bhattacharjee, S. 2011. Sites of generation and physicochemical basis of formation of reactive oxygen species in plant cell. pp. 1-30. In: Dutta Gupta, S. (ed.) Reactive Oxygen Species and Antioxidants in Higher Plants. CRC Press, Boca Raton, Florida, USA.
 
 
Bogdanove, A. J., Bauer, D. W., and Beer, S. V. 1998. Erwinia amylovora secretes DspE, a pathogenicity factor and functional AvrE homolog, through the Hrp (type III secretion) pathway. Journal of Bacteriology 180: 2244-2247.
 
 
Bonasera, J. M., Meng, X., Beer, S. V., Owens, T., and Kim, W. S. 2006. Interaction of DspE/A, a pathogenicity/avirulence protein of Erwinia amylovora, with pre-ferredoxin from apple and its relationship to photosynthetic efficiency. Acta Horticulturae 704: 473-477.
 
 
Boureau, T., Siamer, S., Perino, C., Gaubert, S., Patrit, O., Degrave, A., Fagard, M., Chevreau, E., and Barny, M. A. 2011. The HrpN effector of Erwinia amylovora, which is involved in type III translocation, contributes directly or indirectly to callose elicitation on apple leaves. Molecular Plant Microbe Interaction 24: 577-584.
 
 
DebRoy, S., Thilmony, R., Kwack, Y. B., Nomura, K., and He, S. Y. 2004. A family of conserved bacterial effectors inhibits salicylic acid-mediated basal immunity and promotes disease necrosis in plants. Proceedings of the National Academy of Sciences of the USA 101: 9927-9932.
 
 
Degrave, A., Fagard, M., Perino, S., Brisset, M. N., Gaubert, S., Laroche, S., Patrit, O., and Barny, M. A. 2008. Erwinia amylovora type three-secreted proteins trigger cell death and defense responses in Arabidopsis thaliana. Molecular Plant Microbe Interaction 21: 1076-1086.
 
 
Dias, C. V., Mendes, J. S., Dos Santos, A. C., Pirovani, C. P., Da Silva Gesteira, A., Micheli, F., Gramacho, K. P., Hammerstone, J., Mazzafera, P., and De Mattos Cascardo, J. C. 2011. Hydrogen peroxide formation in cacao tissues infected by the hemibiotrophic fungus Moniliophthora perniciosa. Plant Physiology and Biochemistry 49: 917-922.
 
 
Durrant, W. E., and Dong, X. 2004. Systemic acquired resistance. Annual Review of Phytopathology 42: 185-209.
 
 
Erfaninia, K., Abdollahi, H., and Khosroshahli, M. 2014. Effect of several chloroplast electron transport chain inhibitors on interaction of Erwinia amylovora with susceptible and tolerant apple and pear cultivars. Plant Pathology 49: 201- 214 (in Persian).
 
 
Erfaninia, K., Ghahremani, Z., and Abdollahi, H. 2013. Are chloroplasts key organelles for determining susceptibility of apple and pear genotypes to fire blight? Acta Horticulturae 1056: 295-305.
 
Ghahremani, Z. 2009. Evaluation of electron transport chain inhibitors and systemic acquired resistance (SAR) activator on fire blight development of host Plant. MSc. Thesis, Science and Research Branch, IslamicAzad University, Tehran, Iran. (in Persian).
 
 
Kim, J. F., and Beer, S. V. 1995. HrpW of Erwinia amylovora, a new harpin that contains a domain homologous to pectatelyases of a distinct class. Journal of Bacteriology 180: 5203-5210.
 
 
Kong, X., and Li, D. 2011. Hydrogen peroxide is not involved in HrpN from Erwinia amylovora-induced hypersensitive cell death in maize leaves. Plant Cell Reports 30: 1273.
 
 
Launay, A., Patrit, O., Wenes, E., and Fagard, M. 2016. DspA/E contributes to apoplastic accumulation of ROS in non host A. thaliana. Frontiers in Plant Science 7: 545.
 
 
Leblay, C., Chevrau, E., and Raboin, L. M. 1991. Adventitious shoot regeneration from in vitro leaves of several pear cultivars (Pyrus communis L.). Plant Cell Tissue and Organ Culture 25: 99-105.
 
 
 
Norelli, J. L., Jones, A. L., and Aldwinckle, H. S. 2003. Fire blight management in the twenty-first century: using new technologies that enhance host resistance in apple. Plant Disease 87: 756-765.
 
 
Oh, C. S., and Beer, S. V. 2005. Molecular genetics of Erwinia amylovora involved in the development of fire blight. FEMS Microbiology Letters 253: 185-192.
 
 
 
Rolke, Y., Liu, S., Quidde, T., Williamson, B., Schouten, S., Welting, K. M., Sievers, V., Tudzynski, B., and Tudzynski, P. 2004. Functional analysis of H2O2-generating systems in B. cinerea: The major Cu-Zn SOD (BCSOD1) has impact on virulence on bean, whereas a glucose oxidase (BCGOD1) is dispensable. Molecular Plant Pathology 5: 17-27.
 
 
Thordal-Christensen, H., Zhang, Z., Wei, Y., and Collinge, D. B. 1997. Subcellular localization of H2O2 in Plants: H2O2 accumlation in papillae and hypersensitive response during the burley–powdery mildew interaction. The Plant Journal 11: 1187-1194.
 
 
Venisse, J. S., Barny, M. A., Paulin, J. P., and Brisset, M. N. 2003. Involvement of three pathogenicity factors of Erwinia amylovora in the oxidative stress associated with compatible interaction in pear. FEBS Letter 537: 198-202.
 
 
Venisse, J. S., Malnoy, M., Faize, M., Paulin, J. P., and Brisset, M. N. 2001. Modulation of defense responses of Malus spp. during compatible and incompatible interactions with Erwinia amylovora. Molecular Plant Microbe Interaction 15: 1204-1212.
 
 
Wei, Z. M., Laby, R. J., Zumoff, C. H., Bauer, D. W., He, S. Y., Collmer, A., and Beer, S. V. 1992. Harpin, elicitor of the hypersensitive response produced by the plant pathogen Erwinia amylovora. Science 257: 85-88.
 
 
Xie, Z., and Chen, Z. 2000. Harpin induced hypersensitive cell death is associated with altered mitochondrial functions in tobacco cells. Molecular Plant Microbe Interaction 13: 183-190.
 
 
Yabuta, Y., Mieda, T., Rapolu, M., Nakamura, A., Motoki, T., Maruta, T., Yoshimura, K., Ishikawa, T., and Shigeoka, S. 2007. Light regulation of ascorbate biosynthesis is dependent on the photosynthetic electron transport chain but independent of sugars in Arabidopsis. Journal of Experimental Botany 58: 2661-2671.
 
 
Zhao, Y. F., and Qi, M. 2011. Comparative genomics of Erwinia amylovora and related Erwinia species-What do we learn? Genes 2: 627-639.
 
 
Seed and Plant Journal
Volume 33, Issue 3 - Serial Number 3
November 2018
Pages 315-333

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