Phenotypic and Molecular Evaluation of some Advanced and Elite Bread Wheat Lines to Pyrenophora tritici-repentis, the Causal Agent of Tan Spot Disease

Document Type : Research Paper

Authors

Seed and Plant Improvement Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran.

Abstract

In this study, Advanced Regional Wheat Yield Trials (ARWYT) and Elite Regional Wheat Yield Trials (ERWYT) lines of Northern warm and humid zone were evaluated against Pyrenophora tritici-repentis isolate P8 (race 8), the causal agent of tan spot. Wheat lines were planted in three replications and seedlings were inoculated with the conidia of the isolate at two-leaf stage. Cultivars BR34 and Grandin were used as resistant and susceptible checks, respectively. Evaluation of ERWYT lines of year 2012 (N=20) and 2013 (N=20) showed that only one line ER-N-91-15 (year 2012) and four lines ER-N-92-5, ER-N-92-9, ER-N-92-12 and ER-N-92-20 (year 2013) were resistant to P8 isolate and the remaining lines were moderately susceptible, susceptible or highly susceptible. Furthermore, evaluation of ARWYT lines of year 2013 (AR-N-92) showed that out of 40 lines, only line AR-N-92-39 was resistant to P8 isolate. In addition, the reaction of these lines to ToxA infiltration were evaluated along with PCR screening for the presence or absence of the susceptibility gene, Tsn1. In total, 19 lines were recorded as susceptible to ToxA infiltration assay. PCR screening using gene specific primers showed that the necrosis observed after ToxA infiltration is absolutely correlated with the presence of Tsn1. Generally, the results of this study revealed that most of the wheat genotypes are susceptible to this disease, which is mostly due to lack of screening to the disease during breeding programs.

Highlights

Anderson, J. A., Effertz, R. J., Faris, I. D., Franel, L. J., Meinhardt, S. W., and Gill, B. S. 1999. Genetic analysis of sensitivity to Pyrenophora tritici- repentis necrosis- inducing toxin in durum and common wheat. Phytopathology 89: 293-297.

 

 

Anonymous 2016. Food Outlook: Biannual Report on Global Food Markets. Food and Agriculture Organization of the United Nations (FAO), Rome, Italy. 135 pp.

 

 

Chu, C. G., Friesen, T. L., Faris, J. D., and Xu, S. S. 2008. Evaluation of seedling resistance to tan spot and Stagonospora nodorum blotch in tetraploid wheat. Crop Science 48: 1107-1116.

 

 

Ciuffetti, L. M., and Tuori, R. P. 1999. Advances in the characterization of the ‎Pyrenophora tritici-repentis-wheat interaction. Phytopathology‎‏ ‏‎89: 444-449.‎

 

 

De Wolf, E. D., Effertz, R. J., Ali, S., and Francl, L. J. 1998. Vistas of tan spot research. Canadian Journal of Plant Pathology 20: 349-370.

 

 

Duveiller, E., Kandel, Y. R., Sharma, R. C., and Shrestha, S. M. 2005. Epidemiology of foliar blights (spot blotch and tan spot) of wheat in the plains bordering the Himalayas. Phytopathology 95: 48-256.

 

 

Eyal, Z., and Levy, E. 1987. Variations in pathogenicity patterns of Mycosphaerella graminicola within ‎Triticum spp. in Israel. Euphytica 36: 237-250.‎

 

 

Faris, J. D., Zhang, H. Lu., Reddy, S., Cloutier, J. P., Fellers, S. W., Meinhardt, J. B., Rasmussen, S. S., Xu, R. P., Oliver, K. J., and Friesen, T. L. 2010. A unique wheat disease resistance-like gene governs effector-triggered susceptibility to nectotrophic pathogens. Proceedings of the National Academy of Sciences, USA 107: 13544-13549.

 

 

Lamari, L., and Bernier, C. C. 1989a. Virulence of isolates of Pyrenophora tritici-repentis on 11 wheat cultivars and cytology of differential host reactions. Canadian Journal of Plant Pathology 11: 284-290.

 

 

Lamari, L., and Bernier, C. C. 1989b. Evaluation of wheat reaction to tan spot (Pyrenophora tritici-repentis) based on lesion type. Canadian Journal of Plant Pathology 11:49-56.

 

 

Lamari, L., and Bernier, C. C. 1991. Genetics of tan necrosis and extensive chlorosis in tan spot of wheat caused by Pyrenophora tritici-repentis. Phytopathology 81: 1092-1095.

 

 

Lamari, L., and Strelkov, S. E. 2010. The wheat/Pyrenophora tritici-repentis interaction: progress towards an understanding of tan spot disease. Canadian Journal of Plant Pathology 32: 4-10.

 

 

Lamari, L., Bernier, C. C., and Smith, R. B. 1991. Wheat genotypes that develop both tan necrosis and extensive chlorosis in response to isolates of Pyrenophora tritici-repentis. Plant Disease 75: 121-2.

 

 

Lamari, L., Gilbert, J., and Tekauz, A. 1998. Race differentiation in Pyrenophora tritici-repentis and survey of physiologic variation in western Canada. Canadian Journal of Plant Pathology 20: 396-400.

 

 

Lamari, L., Sayoud, R., Boulif, M., and Bernier, C. C. 1995. Identification of a new race in Pyrenophora tritici-repentis: implications for the current pathotype classification system. Canadian Journal of Plant Pathology 17: 312-318.

 

 

Lamari, L., Strelkov, S. E., Yahyaoui, A., Orabi, J., and Smith, R. B. 2003. The identification of two new races of Pyrenophora tritici-repentis from the host center of diversity confirms a one-to-one relationship in tan spot of wheat. Phytopathology 93: 391-396.

 

 

Mehrabi, R., Karami, N., and Torabi, M. 2015. The presence of the susceptibility gene Tsn1, in some wheat lines and their reactions to ToxA infiltration produced by Pyrenophora tritici-repentis. Iranian Journal of Plant Protection Science 49: 87-94.

 

 

Postnifova, E. N., and Khashanova, B. A. 1998. Tan spot in central Asia. pp. 107-113. In: Duveiller, E., Dubin, H. J., Reeves, J. Y., and McNab, A. (eds.) Helminthosporium Blights of Wheat: Spot Blotch and Tan spot. CIMMYT, Mexico, D. F. Mexico.

 

 

Rajabpour, M., Mehrabi, R., Torabi, M., and Ebrahimi, A. 2014. Distribution and frequency to toxin producing genes (ToxA and ToxB) in populations of Pyrenophora tritici-repentis, the causal agent of wheat tan spot in north of Iran. Seed and Plant Improvement Journal 30-1: 73-83 (in Persian).

 

 

Rees, R. G., Platz, G. J., and Mayer, R. J. 1988. Susceptibility of Australian wheats to Pyrenophora tritici-repentis. Journal of Agricultural Research 39: 141-51.

 

 

Saghai-Maroof, M. A., Soliman, K. M., Jorgensen, R. A., and Allard, R. W. 1984. Ribosomal DNA sepacer-length polymorphism in barley: Mendelian inheritance, chromosomal location, and population dynamics. Proceedings of the National Academy of Science, USA. 81: 8014-8019.

 

 

Shabeer, A., and Bockus, W. W. 1988. Tan spot effects on yield and yield components relative to growth stage in winter wheat. Plant Disease 72: 599-602.

 

 

Siedler, H., Obst, A., Hsam, S. L. K., and Zeller, F. J. 1994. Evaluation for resistance to Pyrenophora tritici-repentis in Aegilops tauschii Coss. And synthetic hexaploid wheat amphiploids. Genetic Resources and Crop Evaluation 41: 27-34.

 

 

Singh, P. K., and Hughes, G. R. 2006. Genetic similarity among isolates of Pyrenophora tritici-repentis, causal agent of tan spot of wheat. Phytopathology 154: 178-184.

 

 

Singh, P. K., Mergoum, M., Ali, S., Adhikari, T. B., and Hughes, G. R. 2008a. Genetic analysis of resistance to Pyrenophora tritici-repentis races 1 and 5 in tetraploid and hexaploid wheat. Phytopathology 98: 702-708.

 

 

Singh, P. K., Mergoum, M., Ali, S., Adhikari, T. B., Elias, E. M., Anderson, J. A., Glover, K. D., and Berzonsky, W. A. 2006. Evaluation of elite wheat germplasm for resistance to tan spot. Plant Disease 90: 1320-1325.

 

 

Singh, P. K., Mergoum, M., Ghavami, F., Ali, S., Adhikari, T. B., and Kianian, S. F. 2008b. Genetic analysis and mapping of tan spot resistance genes using DArT markers. Phytopathology 98: S147.

 

 

Sivanesan, A. 1987. Graminicolous species of Bipolaris Curvularia, Drechslera, Exserohilum and their teleomorphs. CAB International Mycological Institute, Mycological paper 158: 261 pp.

 

 

Strelkov, S. E., and Lamari, L. 2003. Host-parasite interaction in tan spot (Pyrenophora tritici-repentis) of wheat. Canadian Journal of Plant Pathology 25: 339-349.

 

 

Sykes, E. E., and Bernier, C. C. 1991. Qualitative inheritance of tan spot resistance in hexaploid, tetraploid, and diploid wheat. Canadian Journal of Plant Pathology 13: 38-44.

 

 

Tadesse, W., Hsam, S. L. K., Wenzel, G., and Zeller, F. J. 2006a. Identification and monosomic analysis of tan spot resistance genes in synthetic wheat lines (Triticum turgidum L. × Aegilops tauschii Coss.). Crop Science 46: 1212–1217.

 

 

Tadesse, W., Hsam, S. L. K., and Zeller, F. J. 2006b. Evaluation of common wheat cultivars for tan spot resistance and chromosomal location of a resistance gene in the cultivar Salamouni. Plant Breeding 125: 318-322.

 

 

Xu, S. S., Friesen, T. L., and Mujeeb-Kazi, A. 2004. Seedling resistance to tan spot and Stagonospora ‎nodorum blotch in synthetic hexaploid wheats. Crop Science 44: 2238-2245.‎

 

 

 

Keywords

References

Anderson, J. A., Effertz, R. J., Faris, I. D., Franel, L. J., Meinhardt, S. W., and Gill, B. S. 1999. Genetic analysis of sensitivity to Pyrenophora tritici- repentis necrosis- inducing toxin in durum and common wheat. Phytopathology 89: 293-297.
 
 
Anonymous 2016. Food Outlook: Biannual Report on Global Food Markets. Food and Agriculture Organization of the United Nations (FAO), Rome, Italy. 135 pp.
 
 
Chu, C. G., Friesen, T. L., Faris, J. D., and Xu, S. S. 2008. Evaluation of seedling resistance to tan spot and Stagonospora nodorum blotch in tetraploid wheat. Crop Science 48: 1107-1116.
 
 
Ciuffetti, L. M., and Tuori, R. P. 1999. Advances in the characterization of the ‎Pyrenophora tritici-repentis-wheat interaction. Phytopathology‎‏ ‏‎89: 444-449.‎
 
 
De Wolf, E. D., Effertz, R. J., Ali, S., and Francl, L. J. 1998. Vistas of tan spot research. Canadian Journal of Plant Pathology 20: 349-370.
 
 
Duveiller, E., Kandel, Y. R., Sharma, R. C., and Shrestha, S. M. 2005. Epidemiology of foliar blights (spot blotch and tan spot) of wheat in the plains bordering the Himalayas. Phytopathology 95: 48-256.
 
 
Eyal, Z., and Levy, E. 1987. Variations in pathogenicity patterns of Mycosphaerella graminicola within ‎Triticum spp. in Israel. Euphytica 36: 237-250.‎
 
 
Faris, J. D., Zhang, H. Lu., Reddy, S., Cloutier, J. P., Fellers, S. W., Meinhardt, J. B., Rasmussen, S. S., Xu, R. P., Oliver, K. J., and Friesen, T. L. 2010. A unique wheat disease resistance-like gene governs effector-triggered susceptibility to nectotrophic pathogens. Proceedings of the National Academy of Sciences, USA 107: 13544-13549.
 
 
Lamari, L., and Bernier, C. C. 1989a. Virulence of isolates of Pyrenophora tritici-repentis on 11 wheat cultivars and cytology of differential host reactions. Canadian Journal of Plant Pathology 11: 284-290.
 
 
Lamari, L., and Bernier, C. C. 1989b. Evaluation of wheat reaction to tan spot (Pyrenophora tritici-repentis) based on lesion type. Canadian Journal of Plant Pathology 11:49-56.
 
 
Lamari, L., and Bernier, C. C. 1991. Genetics of tan necrosis and extensive chlorosis in tan spot of wheat caused by Pyrenophora tritici-repentis. Phytopathology 81: 1092-1095.
 
 
Lamari, L., and Strelkov, S. E. 2010. The wheat/Pyrenophora tritici-repentis interaction: progress towards an understanding of tan spot disease. Canadian Journal of Plant Pathology 32: 4-10.
 
 
Lamari, L., Bernier, C. C., and Smith, R. B. 1991. Wheat genotypes that develop both tan necrosis and extensive chlorosis in response to isolates of Pyrenophora tritici-repentis. Plant Disease 75: 121-2.
 
 
Lamari, L., Gilbert, J., and Tekauz, A. 1998. Race differentiation in Pyrenophora tritici-repentis and survey of physiologic variation in western Canada. Canadian Journal of Plant Pathology 20: 396-400.
 
 
Lamari, L., Sayoud, R., Boulif, M., and Bernier, C. C. 1995. Identification of a new race in Pyrenophora tritici-repentis: implications for the current pathotype classification system. Canadian Journal of Plant Pathology 17: 312-318.
 
 
Lamari, L., Strelkov, S. E., Yahyaoui, A., Orabi, J., and Smith, R. B. 2003. The identification of two new races of Pyrenophora tritici-repentis from the host center of diversity confirms a one-to-one relationship in tan spot of wheat. Phytopathology 93: 391-396.
 
 
Mehrabi, R., Karami, N., and Torabi, M. 2015. The presence of the susceptibility gene Tsn1, in some wheat lines and their reactions to ToxA infiltration produced by Pyrenophora tritici-repentis. Iranian Journal of Plant Protection Science 49: 87-94.
 
 
Postnifova, E. N., and Khashanova, B. A. 1998. Tan spot in central Asia. pp. 107-113. In: Duveiller, E., Dubin, H. J., Reeves, J. Y., and McNab, A. (eds.) Helminthosporium Blights of Wheat: Spot Blotch and Tan spot. CIMMYT, Mexico, D. F. Mexico.
 
 
Rajabpour, M., Mehrabi, R., Torabi, M., and Ebrahimi, A. 2014. Distribution and frequency to toxin producing genes (ToxA and ToxB) in populations of Pyrenophora tritici-repentis, the causal agent of wheat tan spot in north of Iran. Seed and Plant Improvement Journal 30-1: 73-83 (in Persian).
 
 
Rees, R. G., Platz, G. J., and Mayer, R. J. 1988. Susceptibility of Australian wheats to Pyrenophora tritici-repentis. Journal of Agricultural Research 39: 141-51.
 
 
Saghai-Maroof, M. A., Soliman, K. M., Jorgensen, R. A., and Allard, R. W. 1984. Ribosomal DNA sepacer-length polymorphism in barley: Mendelian inheritance, chromosomal location, and population dynamics. Proceedings of the National Academy of Science, USA. 81: 8014-8019.
 
Shabeer, A., and Bockus, W. W. 1988. Tan spot effects on yield and yield components relative to growth stage in winter wheat. Plant Disease 72: 599-602.
 
 
Siedler, H., Obst, A., Hsam, S. L. K., and Zeller, F. J. 1994. Evaluation for resistance to Pyrenophora tritici-repentis in Aegilops tauschii Coss. And synthetic hexaploid wheat amphiploids. Genetic Resources and Crop Evaluation 41: 27-34.
 
 
Singh, P. K., and Hughes, G. R. 2006. Genetic similarity among isolates of Pyrenophora tritici-repentis, causal agent of tan spot of wheat. Phytopathology 154: 178-184.
 
 
Singh, P. K., Mergoum, M., Ali, S., Adhikari, T. B., and Hughes, G. R. 2008a. Genetic analysis of resistance to Pyrenophora tritici-repentis races 1 and 5 in tetraploid and hexaploid wheat. Phytopathology 98: 702-708.
 
 
Singh, P. K., Mergoum, M., Ali, S., Adhikari, T. B., Elias, E. M., Anderson, J. A., Glover, K. D., and Berzonsky, W. A. 2006. Evaluation of elite wheat germplasm for resistance to tan spot. Plant Disease 90: 1320-1325.
 
 
Singh, P. K., Mergoum, M., Ghavami, F., Ali, S., Adhikari, T. B., and Kianian, S. F. 2008b. Genetic analysis and mapping of tan spot resistance genes using DArT markers. Phytopathology 98: S147.
 
 
Sivanesan, A. 1987. Graminicolous species of Bipolaris Curvularia, Drechslera, Exserohilum and their teleomorphs. CAB International Mycological Institute, Mycological paper 158: 261 pp.
 
 
Strelkov, S. E., and Lamari, L. 2003. Host-parasite interaction in tan spot (Pyrenophora tritici-repentis) of wheat. Canadian Journal of Plant Pathology 25: 339-349.
 
 
Sykes, E. E., and Bernier, C. C. 1991. Qualitative inheritance of tan spot resistance in hexaploid, tetraploid, and diploid wheat. Canadian Journal of Plant Pathology 13: 38-44.
 
 
Tadesse, W., Hsam, S. L. K., Wenzel, G., and Zeller, F. J. 2006a. Identification and monosomic analysis of tan spot resistance genes in synthetic wheat lines (Triticum turgidum L. × Aegilops tauschii Coss.). Crop Science 46: 1212–1217.
 
 
Tadesse, W., Hsam, S. L. K., and Zeller, F. J. 2006b. Evaluation of common wheat cultivars for tan spot resistance and chromosomal location of a resistance gene in the cultivar Salamouni. Plant Breeding 125: 318-322.
 
 
Xu, S. S., Friesen, T. L., and Mujeeb-Kazi, A. 2004. Seedling resistance to tan spot and Stagonospora ‎nodorum blotch in synthetic hexaploid wheats. Crop Science 44: 2238-2245.‎
 
 
Seed and Plant Journal
Volume 33, Issue 2
August 2017
Pages 265-282

Files

Share

How to cite

Statistics