Susceptibility to Chlorosis and Its Relationship to Leaf Iron Contents in some Genotypes of Quince (Cydonia oblonga Mill.)

Document Type : Research Paper

Authors

1 Horticultural Science Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran.

2 College of Agriculture and Natural Resources, Research and Science Branch of Tehran, Islamic Azad University, Tehran, Iran.

Abstract

One of the difficulties of quince cultivation and growing in Iran is calcareous soils which cause leaf chlorosis. This phenomenon appears as leaf yellowish and causes loss of the leaf efficiency and yield of tree. The current research was conducted to evaluate the variation of leaf chlorophyll content and its relationship to the iron contents in the leaves of quince genotypes to select more tolerant ones. Thirty genotypes from Isfahan, Khorasan Razavi and Guilan provinces were evaluated for chlorophyll contents in the leaves of the upper and lower parts of the shoots. The results showed the range of 3.8 to 8.9 mgg-1 of fresh weight for total chlorophyll in the genotypes. The highest quantity of chlorophylls was in July, followed by a reduction in the chlorophyll contents of the majority of genotypes, but the rate of increase was higher for chl b, that caused diminution of chl a/b ratio from 2 and higher to about 1.1 in the mid-summer. The highest level of tolerance to the leaf chlorosis was observed in two genotypes from Khorasan Razavi, encoded as M3 and M4, while genotype KM1 from Isfahan was extremely susceptible. Evaluation of iron contents and chlorosis of the leaves of quince genotypes did not show significant correlation between these traits, not only for the chlorophyll contents of different months, but also for the mean chlorophyll contents of entire growth season.

Keywords


Abd El-Razek, E., and Saleh, M. M. S. 2012. Improve productivity and fruit quality of florida prince peach trees using foliar and soil applications of amino acids. Middle-East Journal of Scientific Research 12: 1165-1172.
 
 
Abdollahi, H., Alipour, M., Khorramdel Azad, M., Mehrabipour, S., Ghasemi, A., Adli, M., Atashkar, D., and Akbari, M. 2011. Establishment of quince (Cydonia oblonga Mill.) germplasm collection from various regions of Iran. Acta Horticulturae 976: 199-203.
 
 
Abdollahi, H., Ghasemi, A., and Mehrabipour, S. 2008. Evaluation of fire blight resistance in some quince (Cydonia oblonga Mill.( genotypes. II. Resistance of genotypes to the disease. Seed and Plant 24: 529-541 (in Persian).
 
 
Abdollahi, H., Ghasemi, A., and Mehrabipour, S. 2010. Interaction effects of rootstock and genotype on tolerance to iron deficiency chlorosis in some quince (Cydonia oblonga Mill.) genotypes from central regions of Iran. Seed and Plant Improvement Journal 26-1: 1-14 (in Persian).
 
 
Alcantara, E., Montilla, I., Ramirez, P., Garcia-Molina, P., and Romera, F. J. 2012. Evaluation of quince clones for tolerance to iron chlorosis on calcareous soil under field conditions. Scientia Horticulturae 138: 50–54.
 
 
Alipour, M., Abdollahi, H., Abdousi, V., Ghasemi, A. A., Adli, M., and Mohamadi, M. 2014. Evaluation of vegetative and reproductive characteristics and distinctness of some quince (Cydonia oblonga Mill.) genotypes from different regions of Iran. Seed and Plant Improvement Journal 30-1: 507-529 (in Persian).
 
 
Anonymous 2015. FAO Statistics for Perennial Crops and Fruits. FAO Publication, Rome, Italy.
 
 
Asadi Kangarshahi, A., Akhlaghi Amiri, N., and Samar, M. 2015. Possibility of using chlorosis degree and active iron (Fe2+) to assess the tolerance of some citrus rootstocks to calcareous soils. Iranian Journal of Soil Research 29: 269-284 (in Persian).
 
 
Bell, L. R., and Leitao, M. J. 2011. Cydonia. pp. 1-16. In: Chittaranjan, K. (ed.). Wild Crop Relatives: Genomic and Breeding Resources. Springer-Verlag, Berlin, Heidelberg, Germany.
 
 
Beneragama, C. K., and Goto, K. 2010. Chlorophyll a:b ratio increases under low-light in ‘Shade-tolerant’ Euglena gracilis. Tropical Agricultural Research 22: 12-25.
 
 
Bienfait, H. F., and van der Mark, F. 1983. Phytoferritin and its role in iron metabolism. pp. 111–123. In: Robb, D. A., and Pierpoint, W. S. (eds.). Metals and Micronutrients. Uptake and Utilization by Plants. Academic Press, London, UK.
 
 
Camejo, D., and Torres, W. 2001. High temperature effect on tomato (Lycopersicon esculentum) pigment and protein content and cellular viability. Cultivos Tropicales 22: 13-17.
 
 
Çelik, H., and Katkat, A. V. 2007. Some parameters in relation to iron nutrition status of peach orchards. Journal of Biology and Environmental Science 1: 111-115.
 
 
Cui, L., Li, J., Fan, Y., Xu, Sh., and Zhang, Z. 2006. High temperature effects on photosynthesis, PSII functionality and antioxidant activity of two Festuca arundinacea cultivars with different heat susceptibility. Botanical Studies 47: 61-69.
 
 
Dolcet-Sanjuan, R., Mok, D. W. S., and Mok, M. C. 1992. Characterization and in vitro selection for iron efficiency in Pyrus and Cydonia. In Vitro Cellular and Developmental Biology-Plant 28: 25–29.
 
 
Ghasemi, A., Nassiri, J., and Yahyaabadi, M. 2010. Study of the relative tolerance of quince (Cydonia oblonga Mill.) rootstocks to different bicarbonate concentrations. Seed and Plant Production Journal 26-2: 137-151 (in Persian).
 
 
Hagin, J., and Tucker, B. 1982. Fertilization of Dryland and Irrigated Soils. Springer-Verlag, New York, USA. 186po.
 
 
Haisman, D. R., and Clarke, M. W. 1975. The interfacial factor in the heat-induced conversion of chlorophyll to pheophytin in green leaves. Journal of the Science of Food and Agriculture 26: 1111-1126.
 
 
Inskeep, W. P., and Bloom, P. R. 1985. Extraction coefficients of chlorophyll a and b in with N,N'-dimethylformamide and 80% acetone. Plant Physiology 77: 483-485.
 
 
Iravani, F., Baninasab, B., Ghobadi, C., Etemadi, N., Ghasemi, A. A., and Shams, M. 2015. Effect of different rootstocks on vegetative growth and photosynthetic parameters of pear ‘Shahmiveh’ and ‘Natanzi’ cultivars. Journal of Crop Production and Processing 5: 53-63 (in Persian).
 
 
Khorramdel Azad, M., Nassiri, J., and Abdollahi, H. 2013. Genetic diversity of selected Iranian quinces using SSRs from apples and pears. Biochemical Genetics 51: 426–442.
 
 
Khoshgoftarmanesh, A. H., Eshghizadeh, H. R,, Sanaeei Stovar, A., Mirlohi, M. S., and Taban, M. 2013. Reasons for iron chlorosis of plane trees (Plantanus orintalis L.) in green space of Isfahan city, I: Physiological indices of active iron in plant. JWSS - Isfahan University of Technology 17: 19-31 (in Persian).
 
 
Manee, A. 1994. Pear and Quinces Growing. Technical Publishing Corporation of Iran. 113pp. (in Persian).
 
 
Marschner, H. 1995. Mineral Nutrition of Higher Plants. Academic Press, London, UK. 651pp.
 
 
Mehrabipour, S., Abdollahi, H., Hassanzadeh, N., and Ghasemi, A. 2010. The role of some quince stock (Cydonia oblonga) genotypes in susceptibility to fire blight disease. Applied Entomology and Phytopathology 78: 25-42 (in Persian).
 
 
Mengel, K. 1994. Iron availability in plant tissues-iron chlorosis on calcareous soils. Plant and Soil 165: 275-283.
 
 
Mirabdolbaghi, M., and Zarghami, R. 2008. Prognosis of nutrient statuse and vegetative vigor in peach trees by floral analysis. International Journal of Agricultural Biology10: 459-462.
 
 
Neaman, A., and Aguirre, L. 2007. Comparison of different methods for diagnosis of iron deficiency in avocado. Journal of Plant Nutrition 30: 1097-1108.
 
 
Rajabpoor, S., Kiani, S., Sorkheh, K., and Tavakoli, F. 2014. Changes induced by osmotic stress in the morphology, biochemistry, physiology, anatomy and stomatal parameters of almond species (Prunus L. spp.) grown in vitro. Journal of Forestry Research 25: 523−534.
 
 
Reuter, D. J., and Robinson, J. B. 1997. Plant Analysis: An Interpretation Manual. 2nd Edition. CSIRO Publics, Australia.
 
 
Römheld, V., and Nikolic, M., 2007. Iron. pp. 329-350. In: Barker, A.V., and Pilbeam, D.J. (eds.) Handbook of Plant Nutrition. CRC Taylor & Francis Group, Boca Raton, FL, USA.
 
 
Salardini, A. A. 1987. Soil Fertilization. Tehran University Press, University of Tehran, Tehran, Iran. 411pp. (in Persian).
 
 
Samar, S. M., Samavati, S., Tadayon, M. S., Rezai, H., Tehrani, M. M., Ardakani, M. S., Besharati, H., and Fallah, A. R. 2010. Iron in Soil and Plant. Publication of the Iranian Soil and Water Research Institute, Karaj, Iran. 191pp. (in Persian).
 
 
Sonmez, S., and Kaplan, M. 2004. Comparison of various analysis methods for determination of iron chlorosis in apple trees. Journal of Plant Nutrition 27: 2007–2018.
 
 
Takahashi, M., Nakanishi, H., Kawasaki, S., Nishizawa, N. K., and Mori, S. 2001. Enhanced tolerance of rice to low iron-availability in alkaline soils using barley nicotianamine aminotransferase genes. Nature Biotechnology 19: 466-469.
 
 
Tamburini, E., Ferrari, G., Marchetti, M.G., Pedrini, P., and Ferro, S. 2015. Development of FT-NIR models for the simultaneous estimation of chlorophyll and nitrogen content in fresh apple (Malus domestica) leaves. Sensors 15: 2662-2679.
 
 
Terry, N., and Abadia, J. 1986. Function of iron in chloroplasts. Journal of Plant Nutrition 9: 609-646.
 
 
Therios, I., Chouliaras, V., Bizas, D., Boukouvalas, S., Palioura, E., Hatzidimitriou, E., Basioukas, D., Gioldasis, V., and Stavrou, N. 2005. Changes in leaf biochemical and physiological indices due to iron deficiency in citrus. AgroThesis 1: 18-25.