Determinaton of Resistance Sources to Powdery Mildew (Blumeria graminis f.sp. tritici) in Iranian Bread Wheat Germplasm

Document Type : Research Paper

Authors

1 Seed and Plant Improvement Institute

2 Iranian Research Institute of Plant Protection

3 Seed and Plant Improvement Research Department, Golestan Agricultural and Natural Resources Research and Education Center

4 Seed and Plant Improvement Research Department, Ardebil Agricultural and Natural Resources Research and Education Center

5 Seed and Plant Improvement Research Department, Mazandaran Agricultural and Natural Resources Research and Education Center

6 College of Agriculture, Kermanshah Branch, Islamic Azad University, Kermanshah, Iran.

Abstract

A total of 1198 Iranian accessions of Bread Wheat Collection of National Plant Gene Bank of Iran were evaluated and screened for resistance to powdery mildew disease. The genetic materials were evaluated separately in three different sets, each comprising about 400 accessions, in three disease hot spots of Sari, Moghan and Gorgan under natural infection of the disease. The resistant accessions were purified leading to 168 lines which were then evaluated again in three hot spots for resistance under the field condition. Different number of resistant genotypes were determined in each location, however, two genotypes B1G11 (From Khorasan) and B2G6 (From Ilam) were resistant in all three location. The pathogen isolates were collected from different regions and purified by single clones and were inoculated on the differential cultivars. Three pathotypes were determined, all had virulence factors for Pm3b, Pm4a, Pm5, Pm6 and Pm7. The pathotypes lacked virulence factor for Pm2, Pm4b and Pm17. The resistance of 165 genotypes was also evaluated with these three pathotypes at seedling stage in greenhouse. Eighty-nine genotypes were resistant to three pathotypes. Forty-two genotypes had different reaction patterns compared to differential varieties suggesting different resistance gene(s) other than those in differential varieties. The results of this research indicated the potential of Iranian bread wheat germplasm as a source for determination of resistant genotypes to powdery mildew disease.

Keywords

References

References Behrouzin, M., and Foroutan, A. 2003. Study on the role of some factors affecting the epidemics of wheat powdery mildew in Mazandaran province. Seed and Plant 18(4): 450-469 (in Persian). Blanco, A., Gadaleta, A., Cenci, A., Carluccio, A. V., Abdelbacki, A. M., and Simeone, R. 2008. Molecular mapping of the novel powdery mildew resistance gene Pm36 introgressed from Triticum turgidum var. dicoccoides in durum wheat. Theoretical and Applied Genetics 117: 135-142. Chen, X., Luo, Y., Xia, X., Xia, L., Chen, X., Ren, Z., He, Z., and Jia, J. 2005. Chromosomal location of powdery mildew resistance gene Pm16 in wheat using SSR marker analysis. Plant Breeding 124: 225-228. Duan, X., Sheng, B., Zhou, Y., and Xiang, Q. 1998. Monitoring of the virulence population of Erysiphe graminis f. sp. tritici. Acta Phytophylac Sin 25: 31-36. Hao, Y. F., Liu, A. F., Wang, Y. H., Feng, D. S., Gao, J. R., Li, X. F., Liu, S. B., and Wang, H. G. 2008. Pm23: a new allele of Pm4 located on chromosome 2AL in wheat. Theoretical and Applied Genetics 117: 1205–1212. Hu, X. Y., Ohm, H. W., and Dweikat, I. 1997. Identification of RAPD markers linked to gene Pm1 for resistance to powdery mildew in wheat. Theoretical and Applied Genetetics 94: 832-840. Hua, W., Liu, Z. J., Zhu, J., Xie, C. J., Yang, T. M., Zhou, Y. L., Duan, X. Y., Sun, Q. X., and Liu, Z. Y. 2009. Identification and genetic mapping of Pm42, a new recessive wheat powdery mildew resistance gene derived from wild emmer (Triticum turgidum var. dicoccoides). Theoretical and Applied Genetics 119(2): 223-230. Karimi Jashni, M., Torabi, M., Roustaie, A., Etebarian, H., Okhovat, S., Razavi, M., and Yazdanpanah, F. 2006. Pathotypes of of Blumeria graminis f.sp. tritici the causal agent of powdery mildew from some regions of Iran. Seed and Plant 22(2): 257-271 (in Persian). Karimi Jashni, M., Torabi, M., Roustaee, A., Etebarian, H., Okhovat, S., and Yazdanpanah, F. 2005. Evaluation of resistance of some wheat commercial cultivars and advanced lines to four pathotypes of Blumeria graminis f.sp. tritici in greenhouse. Seed and Plant 21(3): 411-423 (in Persian). Li, G., Fang, T., Zhang, H., Xie, C., Li, H., Yang, T., Nevo, E., Fahima, T., Sun, Q., and Liu, Z. 2009. Molecular identification of a new powdery mildew resistance gene Pm41 on chromosome 3BL derived from wild emmer (Triticum turgidum var. dicoccoides). Theoretical and Applied Genetics 119: 531–539. Liu, Z. Y., Sun, Q.X., Ni, Z. F., Nevo, E., and Yang, T. M. 2002. Molecular characterization of a novel powdery mildew resistance gene Pm30 in wheat originating from wild emmer. Euphytica 123: 21-29. Mains, E. B., and Dietz, S. M. 1930. Physiologic forms of barley mildew Erysiphe graminis f.sp. hordei Marchal. Phytopathology 20: 229-239. McIntosh, R. A., Yamazaki, Y., Dubcovsky, J., Rogers, J., Morris, C., Somers, D. J., Appels R., and Devos, K. M. 2008. Catalogue of gene symbols for wheat. In: Appels, R., Eastwood, R., Lagudah, E., Langridge, P., Mackay, M., McIntyre, L., and Sharp, P. (eds.) Proceedings of the 11th International Wheat Genetics Symposium, Sydney University Press, Sydney, Australia. Mohler, V., Zeller, F. J., Wenzel, G., and Hsam S. L. K. 2005. Chromosomal location of genes for resistance to powdery mildew in common wheat (Triticum aestivum L. em Thell). 9. Gene MlZec1 from the Triticum dicoccoides-derived wheat line Zecoi-1. Euphytica 142: 161-167. Monazzah, M., Torabi, M., Rezaie, S., and Razavi, M. 2008. Pathotypes of Blumeria graminis f.sp. tritici, the causal agent of wheat powdery mildew from some regions of Iran. Seed and Plant 24 (1): 161-176 (in Persian). Monazzah, M., Torabi, M., Rezaie, S., Razavi, M., and Dehghan, M. A. 2009. Evaluation of resistance of some wheat advanced lines to pathotypes of wheat powdery mildew at seedling and adult plant stages. Seed and Plant Improvement Journal 25-1 (1): 33-49 (in Persian). Qiu, Y. C., and Zhang, S. S. 2004. Researches on powdery mildew resistant genes and their molecular markers in wheat. Journal of Triticeae Crops 24: 127–132. Razavi, M., Dehghan, M. A., Safavi, S. A., Barari, H., Torabi, M., Karimi Jashni, M., and Kazemi, H. 2009. Evaluation of the field and seedling resistance of some advanced and elite lines of wheat to Blumeria graminis f.sp. tritici the causal agent of wheat powdery mildew in Iran. Applied Entomology and Phytopathology 77(1): 133-150 (in Persian). Razavi, M., Karimi Jashni, M., Dehghan, M. A., Safavi, S. A., and Barari, H. 2010. Study on the variability for virulence in Blumeria graminis f.sp. tritici cause of wheat powdery mildew using trap nursery in Iran. Applied Entomology and Phytopathology 78(1): 97-106 (in Persian). Rong, J. K., Millet, E., Manisterski, J., and Feldman, M. 2000. A new powdery mildew resistance gene: introgression from wild emmer into common wheat and RFLP-based mapping. Euphytica 115: 121-126. Saari, E. E., and Prescott, J. M. 1975. A scale for appraising the foliar intensity of wheat disease. Plant Disease Reporter 59: 377-380. Salari, M., Yazdani, D., Okhovat, S. M., and Akbari Haghighi, A. 2002. Evaluation of powdery mildew resistance in wheat cultivars in Mazandaran province. Journal of Applied Entomology and Phtopathology 70(1): 25-36 (in Persian). Shannon, C. E. 1948. A mathematical theory of communication. Bell System Technical Journal 27: 379-423. Yahiaoui, N., Srichumpa, P., Dudler, R., and Keller, B. 2004. Genome analysis atdifferent ploidy levels allows cloning of the powdery mildew resistance gene Pm3b from hexaploid wheat. Plant Journal 37: 528-538.
Seed and Plant Journal
Volume 33, Issue 1
November 2017
Pages 45-65

Files

Share

How to cite

Statistics