Effect of Homologous Genomes on Unreduced Gamete Formation in wheat and Aegilops Crosses

Document Type : Research Paper

Authors

1 respectively, Department of Agronomy and Plant Breeding, College of Agriculture, University of Kurdistan, Sanandaj, Iran.

2 Department of Agronomy and Plant Breeding, College of Agriculture, University of Kurdistan, Sanandaj, Iran.

Abstract

Production of unreduced gametes during meiosis is common among interspecific hybrids of plants. In the present study, two cultivars of bread wheat (2n = 6x = 42, AABBDD)were reciprocally crossed with Aegilops triuncialis (2n = 4x = 28; UtUtCtCt) and Ae. cylindrica (2n = 4x = 28; CcCcDcDc) to produce 20 interspecific hybrid combinations. The hypothesis was that unreduced gamete formation in T. aestivum × Aegilops triuncialis (2n = ABDUtCt; which lack a common subgenome) is more than that of T. aestivum × Ae. cylindrica (2n = ABDDcCc) hybrids. This hypothesis was supported by the estimation of unreduced gametes in F1 hybrids. These results showed that lack of homologous genomes in T. aestivum × Ae. Triuncialis led to meiotic restitution in 3% of pollen mother cells (PMCs) resulting in unreduced gamete formation and F2 seed production.

Keywords


Blanco, A., Simeone, R., Tanzarella, O.A., and Greco, B. 1983. Morphology and chromosome pairing of a hybrid between Triticum durum Desf. and Haynaldia villosa (L.) Schur. Theoretical and Applied Genetics 64: 333-7.
 
Cai, X., Xu, S.S., and Zhu, X. 2010. Mechanism of haploidy-dependent unreductional meiotic cell division in polyploid wheat. Chromosoma 119: 275-285.
 
de Storme, N., and Geelen, D. 2013. Sexual polyploidization in plants–cytological mechanisms and molecular regulation. New Phytologist 198: 670-684.
 
de Storme, N., and Mason, A. 2015. Plant speciation through chromosome instability and ploidy change: Cellular mechanisms, molecular factors and evolutionary relevance. Current Plant Biology 1: 10-33.
 
Gu, Y.Q., Coleman-Derr, D., Kong, X., and Anderson, O.D. 2004. Rapid genome evolution revealed by comparative sequence analysis of orthologous regions from four Triticeae genomes. Plant Physiology 135: 459-470.
 
Harlan, J.R., and Zohary, D. 1966. Distribution of wild wheats and barley. Science 153: 1074-1080.
 
Huang, S., Sirikhachornkit, A., Su, X., Faris, J., Gill, B., Haselkorn, R., and Gornicki, P. 2002. Genes encoding plastid acetyl-CoA carboxylase and 3-phosphoglycerate kinase of the Triticum/Aegilops complex and the evolutionary history of polyploidwheat. Proceedings of the National Academy of Sciences of the USA 99: 8133-8138.
 
Islam, A.K.M.R., and Shepherd, K.W. 1980. Meiotic restitution in wheat barley hybrids. Chromosoma 68: 252–261.
 
Lim, K.B., Ramanna, M.S., de Jong, J.H., Jacobsen, E., and van Tuyl, J.M. 2001. Indeterminate meiotic restitution (IMR): a novel type of meiotic nuclear restitution mechanism detected in interspecific lily hybrids by GISH. Theoretical and Applied Genetics 103: 219-230.
 
Loureiro, I., Escorial, C., Garcıa-Baudin, J.M., and Chueca, M.C. 2009. Spontaneous wheat-Aegilops biuncialis, Ae. geniculata and Ae. triuncialis amphiploid production, a potential way of gene transference. Spanish Journal of Agricultural Research 7: 614-620.
 
Maan, S.S., and Sasakuma, T. 1977. Fertility of amphihaploids in Triticinae. Journal of Heredity 57: 76-83.
 
Mason, A. S., Nelson, M., Yan, G., and Cowling, W. 2011. Production of viable male unreduced gametes in Brassicainterspecific hybrids is genotype specific and stimulated by cold temperatures. BMC Plant Biology 11: 103.
 
Mason, A. S., and Pires, J.C. 2015. Unreduced gametes: meiotic mishap or evolutionary mechanism? Trends in Genetics 31: 5-10.
 
Matsuoka, Y., Nasuda, S., Ashida, Y., Nitta, M., Tsujimoto, H., Takumi, S., and Kawahara, T. 2013. Genetic basis for spontaneous hybrid genomedoubling during allopolyploid speciation of common wheat shown by natural variation analyses of the paternal species. PLoS One 8: e68310.
 
Mirzaghaderi, G., and Fathi, N. 2015. Unreduced gamete formation in wheat: Aegilops triuncialis interspecific hybrids leads to spontaneous complete and partial amphiploids. Euphytica 206: 67-75.
 
Petersen, G., Seberg, O., Yde, M., and Berthelsen, K. 2006. Phylogenetic relationships of Triticumand Aegilopsand evidence for the origin of the A, B, and D genomes of commonwheat (Triticum aestivum). Molecular Phylogenetics and Evolution 39: 70-82.
 
Peterson, R., Slovin, J.P., and Chen, C. 2010. A simplified method for differential staining of aborted and non-aborted pollen grains. International Journal of Plant Biology, 1: e13.
 
Ramsey, J. and Schemske, D.W. 1998. Pathways, mechanisms, and rates of polyploid formation in flowering plants. Annual Review of Ecology and Systematics 29: 467-501.
 
Schmidt, A., Schmid, M.W., and Grossniklaus, U. 2015. Plant germline formation: common concepts and developmental flexibility in sexual and asexual reproduction. Development 142: 229-241.
 
Silkova, O.G., Shchapova, A.I., and Shumny, V.K. 2011. Patterns of meiosis in ABDR amphihaploids depend on the specific type of univalent chromosome division. Euphytica 178: 415-426.
 
Tayalé, A., and Parisod, C. 2013. Natural pathways to polyploidy in plants and consequences for genome reorganization. Cytogenetic and Genome Research 140: 79-96.
 
Tiwari, V.K., Rawat, N., Neelam, K., Randhawa, G.S., Singh, K., Chhuneja, P., and Dhaliwal, H.S. 2008. Development of Triticum turgidum subsp. durum – Aegilops longissima amphiploids with high iron and zinc content through unreduced gamete formation in F1 hybrids. Genome 51: 757-766.
 
Wang, C.-J., Zhang, L.-Q., Dai, S.-F., Zheng, Y.-L., Zhang, H.-G., and Liu, D.-C. 2010. Formation of unreduced gametes is impeded by homologous chromosome pairing in tetraploid Triticum turgidum × Aegilops tauschii hybrids. Euphytica 175: 323-329.
 
Xu, S., and Joppa, L. 2000. First‐divisionrestitution in hybrids of Langdon durum disomic substitution lines with rye and Aegilops squarrosa. Plant Breeding 119: 233-241.
 
Xu, S.J., and Dong, Y.S. 1992. Fertility and meiotic mechanisms of hybrids between chromosome autoduplication tetraploid wheats and Aegilops species. Genome, 35: 379-384.
 
Zeng, D.-Y., Hao, M., Luo, J.-T., Zhang, L.-Q., Yuan, Z.-W., Ning, S.-Z., Zheng, Y.-L., and Liu, D.-C. 2014. Amphitelic orientation of centromeres at metaphase I is an important feature for univalent-dependentmeiotic nonreduction. Journal of Genetics 93: 531-534.
 
Zhang, L.-Q., Liu, D.-C., Zheng, Y.-L., Yan, Z.-H., Dai, S.-F., Li, Y.-F., Jiang, Q., Ye, Y.-Q., and Yen, Y. 2010. Frequent occurrence of unreduced gametes in Triticum turgidum–Aegilops tauschii hybrids. Euphytica 172: 285-294.
 
Zhang, L.-Q., Yen, Y., Zheng, Y.-L., and Liu, D.-C. 2007. Meiotic restriction in emmer wheat is controlled by one or more nuclear genes that continue to function in derived lines. Sexual Plant Reproduction 20: 159-166.