Comparison of Total Phenolic Contents of the Leaves and Fruits of Quince Genotypes and its Effects on the Fire Blight Resistance

Document Type : Research Paper

Authors

1 respectively, Faculty of Agriculture and Natural Resources, Abhar Branch, Islamic Azad University, Abhar, Iran.

2 Temperate Fruits Research Center, Horticultural Sciences Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran.

Abstract

Phenols are important secondary metabolites with important role in tolerance to the environmental stresses, and quince leaves and fruits are significant sources of phenolic compounds. The current research was conducted to study the contents and variations of total phenolic compounds in the leaves and fruits tissues of selected quince genotypes from Isfahan and investigate its eventual role in the fire blight resistance in this species.Twelve quinces genotypes, KVD4, SHA1, KVD3, PH2, ET1, NB3, KVD1, KM1, SVS1, SVS2, NB2 and cv. Isfahan as control were evaluated.The ethanol extracts of the leaves and fruits were prepared during spring and summer and total phenol contents were determined by the folin-ciocalteu method, based on equivalent gallic acid. The results showed differences in phenolic compounds in the leaves, of the genotypes, so that genotype KVD4 and cv. Isfahan, with 17.8 and 2.7 mg gallic acid/g fresh weight of the leaves, had the highest and lowest total phenols, respectively. Also a constant increase was observed in the phenol contents of the leaves up to end of the summer. On the other hand, no significant difference was found between the amounts of total phenols in fruits of genotypes. The evaluation of phenol content as major components of non-enzymatic anti-oxidative defense system, against fire blight invasion showed negative correlation of Isv, with phenolic contents of the quince leaves from early summer.

Keywords

References

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Seed and Plant Journal
Volume 32, Issue 3
September 2016
Pages 331-345

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