Conformity of Self- (in) Compatibility Phenotyping and Genotyping Results in some Iranian Apple Cultivars and Genotypes

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Abstract

Apple (Malus domestica Brokh.) as other fruit trees of Rosaceae family is self-incompatible(SI). The apple SI system is controlled by a multi allelic single locus S-locus. In this study, the results of orchard based phenotyping of 25 apple genotypes were compared with the results of on-going S-allele genotyping of them. A more common consensus primer pair, FTQQYQ and FI(D/N)CP(H/R) was able to amplify the S alleles fragments of all samples. Then, using eight pairs of allele specific primers were able to detect S1, S2, S3 and S4 with certain indicating the rather high frequency of these S-alleles in the studied apples. Narsib-e-Mashad, Ardebil2 and also, Englisi-e-Shiraz with three detected S-alleles could be considered as triploids, probably. These primers could not produce any bands for half of the samples leading to apply the remained list of specific primers. Despite of self-incompatibility of apples, some of the cultivars including Payize-e-Mashad, Sheikh Ahmad, Morabbayi, IRI1 and IRI4 showed auto fertility. The genotype IRI6 with highest percentage of fruit set was considerable. According to the results of this research, the PCR based techniques in apple S-genotyping is more useful to recognize cross-compability situation of genotypes.

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References

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Seed and Plant Journal
Volume 31, Issue 3
October 2015
Pages 477-489

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