Descriptive Assessment and Genetic Diversity of some Promising Walnut Genotypes in Zanjan Province

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Abstract

Following the occurrence of spring frost in Khorramdarreh Agro- Industry farm in Zanjan province, Iran, during 2010, fifteen walnut promising genotypes showing frost escape response were selected. Tree, fruit and kernel characteristics of selected genotypes were assayed according IPGRI descriptors. Meanwhile, the genetic diversity, distinction and classification were also assessed using microsatellite markers. The 13 SSR primers produced 37 bands across 15 genotypes, with an average of 2.84 polymorphic fragments per primer. The number of amplified bands varied from 2 to 4 with size of amplicons ranging from 209 to 308 bp. Two loci WGA276 and WGA071 were the highest and lowest distinguishable among genotypes, respectively. Cluster analysis based on both morphological and molecular data clarified the relatively large genetic distance among genotypes. However, clustering of genotypes within the genotypes showed low similarity when morphological and molecular derived dendrograms were compared. Although all evaluated genotypes were appropriate referring to leafing late and producing fruitful buds in terminal and lateral shoots, however, genotype T36R67 generally showed high quality characteristics and is good candidate for further complementary assessments and using in breeding programs.

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Seed and Plant Journal
Volume 30, Issue 4
October 2014
Pages 719-732

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