Identification of Self-Incompatibility Alleles in Iranian Almond Cultivars Using Sequencing

S. A. Mousavi,; M. R. Fatahi Moghaddam,; Z. Zamani,; M. Tatari,; A. Imani,; E. Ortega Pastor,

doi:10.22092/spij.2017.111234

Identification of Self-Incompatibility Alleles in Iranian Almond Cultivars Using Sequencing

Authors

10.22092/spij.2017.111234

Abstract

Identification of self-incompatibility alleles in almond (Prunus dulcis) is a very useful tool for orchard design and for choosing parents in breeding programmes. In order to identify the new S-RNases in some Iranian almond cultivars, each allele fragment was amplified and PCR products were purified. The vector PCR-Blunt-II-TOPO was used for cloning. Constructs were transformed into chemically competent Escherichia coli TOP 10 cells and positive transformants were identified. Plasmids were isolated from positive colonies and sequenced by SECUGEN using M13 primers and then a consensus sequence was obtained for each allele with SeqManII software. The results identified eight alleles in the Iranian almond cultivars including Tejari (S36), Holouei (S37), Sefid (S38), Yazd-17 (S39 and S40), Mashhad 40 (S41), G-R-16 (S42) and Mamaei (S43). Seven of these alleles have not previously been reported in Prunus species, while one of alleles (S40) was similar to that (S5) identified before in an accession of the wild species Prunus webbii. The similarity between self-incompatibility alleles in cultivated almond wild species of Prunus webbii points out the existence of a common ancestor with other Prunus species and also a possible introgression with P. webbii as a primary ancestors in almond evolution.

Keywords