Genetic Diversity in some Plum (Prunus spp.) Cultivars Using Morphological Characteristics and Molecular Markers

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Abstract

Eighteen pulm cultivars including ten Iranian native and eight foreign cultivars were evaluated based on 36 parametric and non-parametric characteristics and 15 RAPD primers. The results of analysis of variance showed significant differences among the cultivars for all characteristics except the petal color. Dissimilarity matrix revealed that two cultivars Mashhad 4 and Dirras-e-Seif and two other cultivars Ontario and Barbang were distinctly different considering their quantitative and qualitative characteristics. Cluster, constructed from morphological data in distance of 25, classified cultivars into two major groups. Cluster analysis could differentiate the Iranian and foreign cultivars in some extents. Molecular data generated a high percentage of polymorphic bands (99.04%), and the similarity matrix of these data showed the highest similarity between Emprofrance and Bokharaye Marghob (71%) and the lowest similarity between Shiro and Bokharaye Mamoli (9%). The cluster constructed from molecular data clustered cultivars from 0.15 to 0.85 genetic similarity coefficient. In the similarity distance of 0.26, the cultivars were divided into three groups where the Dirras-e-Seif cultivar exclusively comprised an indipentent cluster and was distinguished from other cultivars. In valuation of plum cultivars based on morphological and RAPD primers, although Iranian and foreign cultivars were clearly differentiated, but in some cases they showed some differences.

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Seed and Plant Journal
Volume 28, Issue 4
December 2012
Pages 643-661

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