Genetic Diversity of some Iranian Sour Cherry Genotypes Based on Morphological and Molecular Markers

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Abstract

In this study nineteen Iranian sour cherry genotypes from West Azarbaijan, East Azarbaijan, Kordestan, Tehran, Kohgiloye va Boyerahmad, Isfahan, Kerman, Khorasan, Alborz, Fars provinces and Erdi Botermo cultivar from Hungary as control, were evaluated using morphological and molecular markers. In the first experiment, 34 qualitative and 11 quantitative traits were examined. Analysis of variance indicated that genotypes differed significantly for all traits. Factor analysis divided characters into four groups explained 71.86% of total variance. Cluster analysis divided genotypes into four sub groups based on morphological data. The first group consisted of three dwarf genotyps from Yasooj(BO AH V1524), Kordestan(BO KA V282) and Khorasan(BO GA V6442). The second group consisted of one genotype from Tehran(BO LA V181) that had the maximum plant height. In the second experiment, RAPD markers were used for genetic diversity evaluation. Thirteen primers that were used produced 144 bands that 117 of them were polymorphic. Genetic similarity in genotypes ranged from 0.30 to 0.81 . Cluster analysis divided genotypes into six sub groups with 0.54 genetic similarity. In clustering the most of genotypes from one region were grouped together. In this cluster also three dwarfing genotypes BO AH V1524, BO KA V282, and BO LA V181 were grouped together.

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