The cytoplasmic male sterile (CMS) lines that are used in production of hybrid seeds in three-line system often get contaminated with their maintainer lines. Using such impure CMS lines in rice hybrid seed production lead to un-uniformity and yield loss in hybrid rice. Laborious and time consuming phenotypic evaluations are used for elimination of off-type plants in CMS multiplication farms. The other option for off-type elimination in CMS lines is application of molecular markers. In this study, PCR-based mitochondrial markers was used for distinguishing five male sterile lines from five maintainer lines. Results showed that marker cms had ability to differentiate male sterile lines Neda A and Nemat A from their maintainers. Also, marker drrcms differentiated male sterile lines Dasht A, Champa A and Amol 3 A from their respective maintainers. Results of the study demonstrate use of these two markers for off-type detection in CMS seed multiplication process. This is helpful in promotion of hybrid seed production technology in Iran.
G. Kiani, and G. Nematzadeh, (2012). Molecular Purity Assessment of Rice CMS Lines. Seed and Plant Journal, 28(1), 149-153. doi: 10.22092/spij.2017.111097
MLA
G. Kiani, , and G. Nematzadeh, . "Molecular Purity Assessment of Rice CMS Lines", Seed and Plant Journal, 28, 1, 2012, 149-153. doi: 10.22092/spij.2017.111097
HARVARD
G. Kiani, , G. Nematzadeh, (2012). 'Molecular Purity Assessment of Rice CMS Lines', Seed and Plant Journal, 28(1), pp. 149-153. doi: 10.22092/spij.2017.111097
CHICAGO
G. Kiani and G. Nematzadeh, "Molecular Purity Assessment of Rice CMS Lines," Seed and Plant Journal, 28 1 (2012): 149-153, doi: 10.22092/spij.2017.111097
VANCOUVER
G. Kiani, , G. Nematzadeh, Molecular Purity Assessment of Rice CMS Lines. Seed and Plant Journal, 2012; 28(1): 149-153. doi: 10.22092/spij.2017.111097
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