The cytoplasmic male sterile (CMS) lines that are used in production of hybrid seeds in three-line system often get contaminated with their maintainer lines. Using such impure CMS lines in rice hybrid seed production lead to un-uniformity and yield loss in hybrid rice. Laborious and time consuming phenotypic evaluations are used for elimination of off-type plants in CMS multiplication farms. The other option for off-type elimination in CMS lines is application of molecular markers. In this study, PCR-based mitochondrial markers was used for distinguishing five male sterile lines from five maintainer lines. Results showed that marker cms had ability to differentiate male sterile lines Neda A and Nemat A from their maintainers. Also, marker drrcms differentiated male sterile lines Dasht A, Champa A and Amol 3 A from their respective maintainers. Results of the study demonstrate use of these two markers for off-type detection in CMS seed multiplication process. This is helpful in promotion of hybrid seed production technology in Iran.
G. Kiani, , & G. Nematzadeh, (2012). Molecular Purity Assessment of Rice CMS Lines. Seed and Plant Journal, 28(1), 149-153. doi: 10.22092/spij.2017.111097
MLA
G. Kiani; G. Nematzadeh. "Molecular Purity Assessment of Rice CMS Lines". Seed and Plant Journal, 28, 1, 2012, 149-153. doi: 10.22092/spij.2017.111097
HARVARD
G. Kiani, , G. Nematzadeh, (2012). 'Molecular Purity Assessment of Rice CMS Lines', Seed and Plant Journal, 28(1), pp. 149-153. doi: 10.22092/spij.2017.111097
VANCOUVER
G. Kiani, , G. Nematzadeh, Molecular Purity Assessment of Rice CMS Lines. Seed and Plant Journal, 2012; 28(1): 149-153. doi: 10.22092/spij.2017.111097
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