Assessment of Barley Genotypes for Crown Freezing Tolerance and some Physiological Characters

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Abstract

Two experiments were conducted to evaluate 40 barley genotypes for crown freezing tolerance and some physiological traits under controlled conditions. In the first experiment, seeds were planted in pots in the greenhouse and the treatments were arranged as randomized complete block design with three replications. After acclimation, percent of tissue fresh and dry weight, water content and relative water loss were measured in each experimental unit. The second experiment was conducted as split plot design based on randomized complete blocks with three replications. Freezing temperatures were considered as main plots and the genotypes as subplots. After acclimation of the genotypes in the growth chamber, seedling’s crowns were transferred to a freezer and were treated with different freezing temperatures. Then, percent survival of the crowns and consequently LT50 were determined. Analysis of variance of data showed significant differences among genotypes for percent water content of the crown, leaf and seedling and relative water loss. Mean LT50 of the genotypes was -10.140C. Genotypes Ec79-18, A1C84-9, A1C84-14 and Schulyer were more suitable than others in terms of physiological characters. Genotypes EC83-15, EC80-11, EC82-11, A1C84-15, Schulyer and EC79-18 and 3 had the highest crown survival rate and the lowest LT50. The correlation coefficient of percent dry weight of different tissues with percent crown survival were positive and significant. Furthermore, negative significant correlation of percent crown survival with percent leaf and seedling water content and with relative water loss were observed. However, the correlations were not strong.

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Seed and Plant Journal
Volume 28, Issue 1
April 2012
Pages 85-100

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