Micropropagation of some Clonal Rootstocks of Stone Fruits

In the present study propagation of three clonal rootstocks of stone fruits including of VPK1, St-Julien A and GF677 were evaluated with in vitro culture. Explants of apical and lateral buds of rootstocks were disinfected and cultured in modified MS, WPM and Knop media. VPK1 rootstock produced the highest plantlet number and length in WPM and modified MS media. Two other rootstocks produced the highest of plantlet number and length in modified MS medium. VPK1 rootstock in WPM and Knop media and St-Julien A in Knop medium showed chlorosis symptoms, thus combination of plant growth regulators were evaluated in modified MS medium. St-Julien A had the highest proliferation in MS3 medium (containing 1 mgl-1 BAP and 0.5 mgl-1 NAA). Optimum proliferation in GF677 and VPK1 rootstocks were obtained in MS1 medium (containing 0.6 mgl-1 BAP and 0.01 mgl-1 NAA). Evaluating three rootstocks, the highest plantlet length were produced in MS1 medium. VPK1 produced callus in MS2 (containing 0.8 mgl-1 BAP and 0.1 mgl-1 NAA) and MS3 media. Increasing concentration of plant growth regulators led to vitrification and callus production of shoots in GF677 rootstock. St-Julien A and VPK1 rootstocks in medium containing 1 mgl-1 NAA and GF677 rootstock in medium containing 1 mgl-1 IBA produced the highest root length. In rooting medium proliferation of rootstocks were observed. Effect of clonal rootstocks on mean root number was not significant. Rooty rootstocks were transferred to peat and perlite medium in acclimization room.