Virulence Factors of Puccinia triticina the Causal Agent of Wheat Leaf Rust in Different Parts of Iran

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Abstract

In the present study virulence factors of 30 wheat leaf rust isolates were determined in the cereal rusts greenhouses in Seed and Plant Improvement Institute, Karaj, Iran. Rust samples were collected from 13 parts of Iran during 2007. After purification and multiplication of urediniospores, all isolates were separately inoculated on 40 leaf rust near-isogenic lines and maintained in a greenhouse at 22-24˚C. Avirulence/virulence formula was determined for each isolate. The results showed that near-isogenic lines carrying genes Lr3, Lr3bg, Lr14b, Lr20, Lr22a, Lr22b, Lrb, Lr35 and Lr37 were susceptible to all isolates indicating the presence of virulence factors for them in all parts of the country. Virulence factor was not existed for genes Lr9, Lr19, Lr10+, Lr25 and Lr28 in any of the isolates. Genes Lr23+ and Lr2a with 16.6% and 20%, had also the lower

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