Allogamic Characteristics of Rice Cytoplasmic Male Sterile Lines and Using STS Markers for Distinguishing Them

R. Nabavi Wasokolaii,; N. Babaeian Jelodar,; N. Bagheri,; E. Hasannataj,

doi:10.22092/spij.2017.111038

Allogamic Characteristics of Rice Cytoplasmic Male Sterile Lines and Using STS Markers for Distinguishing Them

Authors

10.22092/spij.2017.111038

Abstract

Cytoplasmic male sterile lines for Iranian rice cultivars, Neda, Tarom, Shastak-Mohamadi, Nemat, Onda, Hassani Rishak Ghermez, Amol 3, Dasht, Khazar and Sepidrod were developed after six repeated back crosses generations in the research farm of University of Agricultural and Natural Resources Sciences, Sari, Iran. STS markers (BF-STS-401 and BF-STS-402) were used for distinguishing CMS from maintainer lines or other self-fertile genotypes. The results showed that CMS lines possess two bands (464 and 335 bp) and maintainer lines possess only 335 bp bands. The results of studying allogamic traits indicated that florets of the Iranian CMS lines Sepidrod, Tarom, Amol 3, Dasht, Gerdeh, Hassani Rishak Ghermez and Neda were closed later than those of their male parents. The results also showed that glume openning time had significant positive correlation with filament length (0.720) and stigma length (0.889). Based of the results of the present study, it seems that Iranian CMS lines Neda, Tarom and Sepidrod are more suitable for hybrid seed production due to their favorable and superior floral characteristics.

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