Evaluation of Bread Making Quality of Bread Wheat Cultivars and Lines Using SDS- Sedimentation Volume and High Molecular Weight Glutenin Subunits

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Abstract

Fifty seven advanced breeding lines of hexaploid bread wheat from temperate region trial of Iran were selected and studied for relationship between HMW glutenin subunits and SDS-Sedimentation volume. The SDS-PAGE and SDS-Sedimentation were used for electrophoresis separation and bread making quality respectively. Three subunits at
Glu-A1, five subunits at Glu-B1 and two subunits compositions at Glu-D1 were identified. Results showed that there was a positive and significant correlation between subunit 1 from Glu-A1, 17+18 from Glu-B1 and 5+10 from Glu-D1 with SDS-Sedimentation volume. In the stepwise regression analysis, allele Null at Glu-A1, subunits 2+12 from Glu-D1with negative effect, subunits 13+16 and 17+18 from Glu-B1 with positive effect in high SDS-Sedimentation volume were entered in the model. The results of oneway analysis of variance were also assessed. Subunit 1 at Glu-A1, subunit pairs 13+16 at
Glu-B1 and 5+10 at the Glu-D1 locus had the high means for SDS-Sedimentation volume.

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Seed and Plant Journal
Volume 25, Issue 3
September 2009
Pages 373-383

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