Effects Of Salinity And Nitrogen Nutrition Free-Proline And Oil Content Of Common Pumpkin

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Abstract

In order to study the effects of salinity and nitrogen nutrition on rate of free- praline in the leaves, and effect of salinity on seed oil content of common pumpkin (Cucurbita pepo subsp pepo var. styriaca), the field and greenhouse studies were conducted in Tarbiat Modarres University in 1998 and 1999 cropping seasons. For salinity and nitrogen nutrition effects on rate of free-proline in the leaves, the experimental design was split-plot with RCBD arrangement in three replications in which salinity treatments at four levels (0,2.5,5 and 10 grl-1 of NaCl) were considered as main plots and nitrogen treatments at five levels (0, 75, 150, 225 and 300 kg ha-1 of NH4 N03) as sub-plots. However, for salinity effect on oil content of seed, the experimental design was RCB with three replications. Salinity stress was applied at two stages i.e. by soaking the seeds in the solution for 36 h before planting, and during stage from four leaves until flowering. Meanwhile, nitrogen fertilizer was applied to the plants at three stages (planting, four leaves stage and t1owering). Results indicated that in both years of the experiment, salinity stress increased the amount of free-proline in the leaves compared to the check. The highest rate of proline was obtained when salinity and nitrogen levels were at 2.5 grl-1 and 225 Kgha-1, respectively. The highest oil content in seed was also obtained at salinity of 2.5 grl-1 of NaCl.

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Seed and Plant Journal
Volume 16, Issue 3
September 2000
Pages 359-373

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