Study of haploid production in iranian onions (allium cepa l.) Via in vitro gynogenesis

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Abstract

Iranian onions have good characteristics, but in some of them the yield is lower than F1 cultivars. For this reason, production of hybrid seeds is important. There are many ways for producing inbred lines, but gynogenesis offers the possibility to produce homozygous plant in very short time whereas traditional method takes long time. The aim of this research was to study the responses of six Iranian onion populations, Ghermez-e-Azarshahr, Sefid-e-Kashan, Sefid-e-Kamare-e-Khomain, Sefid-e-Qom, Dorcheh-e-Esfahan, Gholigheseh-e-Zanjan, and two Italian cultivars, Borettana and KLR (used as control) to in vitro gynogenesis on two F6 and JAP media with two replications. The results showed significant differences (P < 0.05) between the two media on percentage of embryo rate, percentage of callus formation and percentage of hyper hydrated flowers and a high interaction (P < 0.05) between media and cultivars. F6medium in comparison with JAP medium had more embryo and callus formation and hyper hydrated flowers. The highest percentage of embryo was produced by Gholigheseh-e-Zanjan onion, while the lowest by Sefid-e-Kamare-e-Khomain.

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Seed and Plant Journal
Volume 16, Issue 3
September 2000
Pages 300-312

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