Determination Of The Most Suitable Culture Medium And Growth Conditions For Micropropagation Of Gf677 (Hybrid Of Almond X Peach) Rootstocks

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Abstract

In the present investigation in vitro propagation of GF677 (Hybrid of almond x peach) rootstock was studied. Explants were isolated from apical and lateral buds early April. Plant materials were then sterilized using Hg Cl2 (0.1%) for 6 minutes. The culture media MS, 1/2 MS and modified Knop (with some changes in microelements) were used in experiments. The best shoot proliferation achieved on modified Knop medium containing 2% sucrose, 1mgll BA, without NAA (0.0). In this medium about 5 shoots per test tube were produced and there were significant difference between hormone concentrates at 0 1% level. The conditions of growth chamber was 16-h light period and a 24/26oC day/night temperature regime. Light intensity was 2500-3000 lux and air humidity was 45%. In rooting stage, use of LS medium containing 0.3 mg/l IBA , 1.6 mg/l thiamin and 7 days darkness period, gave the best results of rooting, up to 80% . Propagated plants via tissue culture were transferred to the soil consisting of 40% peat and 60 % sand mixture, or Jiffy blocks, but Jiffy blocks gave better results

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Seed and Plant Journal
Volume 17, Issue 3
September 2001
Pages 234-243

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