cultivar identificaton and determination of genetic similarity of some bread wheat cultivars grown in iran based on gliadin formulae

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Abstract

Acid PAGE separation was performed on gliadin proteins of 43 bread wheat promissing lines and cultivars grown in Iran. Gliadin formulae were determined based on the electrophoretic mobility and intensity of gliadin components. A total of 58 gliadin components with different electrophoretic mobilities and 53 gliadin electrophoregrams were detected in the bread wheats analysed. The estimation of genetic similarity between 43 bread wheat, using simple matching coefficients (SM), showed a range of similanty coefficients between 0.45-0.98. All the bread wheats were detectable by their gliadin formula. Out of 43 bread wheats analysed, seven were heterogeneous and showed two to three gliadin biotypes. The biotypes differed from each other for at least three components in one region to several components in different electrophoretic regions. Present results confirm that gliadin markers can be considered as a fingerprint to identify unknown samples and intra cultivar heterogeneity of wheats. Besides, catalouges of gliadin formulas of Iranian bread wheats were prepared for the first time. The data obtained in this study can be used to determine the relationship between gliadin components and morphological and agronomical traits in Iranian breadwheat breeding programs.

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Seed and Plant Journal
Volume 19, Issue 3
September 2003
Pages 383-400

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