Regeneration of Plantlets from Fragmented Embryo Explant of Parsi Zira (Bunium persicum Boiss.)

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Abstract

Parsi zira (Bunium persicum Boiss.) that is also called mountainous or black zira, is one of the most important medicinal plants with high economic importance. There is a little information about in vitro culture of Parsi zira. Fragmented embryo was used as an explant in Bunium persicum regeneration. In this method, a great callus induction and regeneration occurred only on the same medium without any subculture. In this research, MS media containing different concentrations of plant growth regulators, NAA and 2,4-D alone or together with Kin, were used. The experiment was conducted using completely randomized design with 30 treatments and 10 replications per treatment. The treatment containing 0.1 mgl-1 NAA and 2 mgl-1 Kin was the best one for regeneration. The treatment containing 0.1 mgl-1 NAA and 0.5 mgl-1 Kin was the best one for somatic embryogenesis. Regeneration occurred in some treatments without kinetin, showing that kinetin is not essential for Bunium persicum regeneration

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Seed and Plant Journal
Volume 24, Issue 3
September 2008
Pages 389-397

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