Effects of Different Sources of Nitrogen on in vitro Growth Characteristics of a Selected Genotype of Wild Cherry (Prunus avium L.)

Authors

Abstract

To study the selective absorption of different nitrogen sources by in vitro shoots of a selected genotype of wild cherry (Prunus avium L.) located in Nour forests in Mazandaran province, Iran, different nitrogen sources in different concentrations of MS medium were used. To optimize proliferation medium, the effect of different nitrogen sources on coefficient of multiplication and quality of micropropagated shoots defined by growth characters was studied. For true to type mass propagation different growth characters like shoot height, inactive leaves percent, total number of leaves, dry weight and coefficient of multiplication in three intervals of 15, 30 and 45 days after culture were analyzed. To understand the effect of nitrogen on biological activities including uptake level, new shoot formation and height, different nitrogen sources including single forms KNO3 and NH4NO3 and the combined form (KNO3+ NH4NO3) of nitrogen in different concentrations were used. These sources were tested in six concentrations including 1.2MS, 1MS, 0.8 MS, 0.6 MS, 0.4 MS, 0.2 MS and control (0.5 MS) independently on growth characters. Comparison of different sources of nitrogen showed that the combined source of nitrogen (KNO3+ NH4NO3) 30 days after culture resulted in the best rates of growth characteristics of the selected wild cherry genotype compared to other nitrogen forms. The optimum concentration range for this form of nitrogen found to be 0.8MS to 1MS compared to the control. As a result, the subculture period was decreased to minimum, and luxury consumption of macronutrient was avoided

Keywords

Seed and Plant Journal
Volume 24, Issue 4
December 2008
Pages 749-764

Files

Share

How to cite

Statistics