Mapping QTLs Controlling Drought Tolerance in a Barley Doubled Haploid population

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Abstract

This research was carried out to find genetic markers linked to drought tolerance in a barley doubled haploid population. In two separate experiments, 158 doubled haploid lines together with their drought tolerant parents (W i2291 and Tadmor) were evaluated in Tel Hadya and Breda (Syria) with 100 millimeter difference in rainfall. They were planted in each environment using an alpha-lattice design with two replications. Linkage map was constructed using 50 SSR and 93 AFLP markers that included 8 linkage groups for the seven chromosomes. QTL analysis was performed using composite interval mapping. Number of QTLs, their genomic location and additive effects were identified between 0.5 and 2.5 gram in Tel Hadya and Breda, respectively. Seven QTLs were determined for grain yield, one of them was common in two environments, and two separate QTLs in mean of environments in each environment. Susceptible stress index (SSI) had three QTLs which two of them were common in controlling grain yield. Bmag13 marker, which is located on third chromosome, had simultaneous linkage with QTLs which control 1000KW, grain yield.

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Seed and Plant Journal
Volume 24, Issue 1
May 2008
Pages 1-15

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