EVALUATION OF SOME TRICHODERMA ISOLATES FOR BIOLOGICAL CONTROL OF CROWN AND ROOT ROT OF WHEAT CAUSED BY BIPOLARIS SPICIFERA (BAINIER) SUBRAM

ETEBARIAN H.R,; ,MOHAMMADIFAR M,

doi:10.22092/spij.2017.110734

EVALUATION OF SOME TRICHODERMA ISOLATES FOR BIOLOGICAL CONTROL OF CROWN AND ROOT ROT OF WHEAT CAUSED BY BIPOLARIS SPICIFERA (BAINIER) SUBRAM

Authors

10.22092/spij.2017.110734

Abstract

Trchoderma harzianum M, T. harzianum T39, T. viride(MO) and T. virens DAR74290 were used as potential biological agents for control of wheat crown and root rot caused by Bipolaris spicifera. Dual culture and volatile test were used for antifungal activity of Trichoderma isolates. Percentage of growth inhibition of Bipolaris spicifera by T. harzianum M, T. harzianum T39, T. viride (MO) and T. virens DAR 74290 were 53.39, 37.37, 33.11 and 40.4 in dual culture and 71.5, 52, 66.5 and 5.64 in volatile test respectively. Results of glasshouse experiments indicated that T. viride (MO) was the most effective. All isolates reduced disease incidence and disease severity in microplot test. There was no significant difference between benomyl and Trichoderma treatments. The effect of benomyl and Trichoderma isolates was similar on reducing the disease. Disease severity in treatment of pathogen plus Trichodermin B , T. viride, T. harzianum varied from 0.74 to 1.63 compared to 3.07 in pathogen control .

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