Study of androgenic ability of f3 populations of barley (hordeum vulgare l.) obtained from crosses between different stress tolerant cultivars

Khosrowchahli, M.; Naghiloo, M.H.; Yousefi, A.; Bakhtyar, F.; Bozorgi Pour, R.

doi:10.22092/spij.2017.110639

Study of androgenic ability of f3 populations of barley (hordeum vulgare l.) obtained from crosses between different stress tolerant cultivars

Authors

10.22092/spij.2017.110639

Abstract

Androgenic ability of microspores of F3 populations obtained from crosses between salinity and heat tolerance (Afzal/Torkman/Kavir), coldand heat tolerance (Boyer/Rojo), salinity tolerance and disease resistance (Ashar/ Hebe), salinity and heat tolerance. (Arigashar/ Matico) and Igri cultivar as control, were studied after pretreatment with 0.3 M mannitol using L1 modified liquid medium supplemented with 3 mgl-1 Phenylacetic acid (PAA), 1 mgl-1 Benzylaminopurin (BAP), 1 mgl-1 Casein and 65 mgl-1 maltose as induction medium. Murashige and Skoog medium supplemented with 1 mgl-1 BAP and 0.5 mgl-1 Naphtalenacetic acid (NAA) was used as regeneration medium. Statistical analysis revealed a significant difference between F3 progenies and Igri cultivar for androgenic ability. Igri cultivar produced on average 566.75 embryos per microspores of 100 anthers and the F3 progenies of Afzal/ Torkman/ Kavir produced on average the highest number of embryos (141.5 embryos/ microspores of 100 anthers) among the progenies of F3 populations. For green plantlet production per microspores of 100 anthers there was also asignificant difference between progenies ofF3 populations and Igri cultivar. For this character Igri cultivar produced the highest number of green plantlets (65.5 plantlets/ microspores of 100anthers) and the progenies of Ashar/ Hebe and Boyer/ Rojo with 18.5 and 17.5 green plantlets respectively per microspores of 100 anthers on average produced more green plantlets than the progenies of Afzal/ Torkman/ Kavir. This means that the mechanisms of embryo induction and green plantlet production may be different. In this study Igri cultivar produced the green plantlet production may be different. In this study Igri cultivar produced the least number of a albino plantlets in comparison with other plant materials

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